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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX30667 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX30667, RRID:AB_625978
- Product name
- NF-M antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Feline
- Host
- Chicken/Avian
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot: 160kDa Neurofilament Medium Antibody [GTX30667] - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with GTX30667, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: 160kDa Neurofilament Medium Antibody [GTX30667] - View of mixed neuron/glial cultures stained with GTX30667 (red). The NF-M protein is assembled into neurofilaments which are found throμghout the axons, dendrites and perikarya of these cells.
