Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [7]
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- Product number
- NBP1-87754 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87754, RRID:AB_11035146
- Product name
- Rabbit Polyclonal NF-M Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human NF-M antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Plasma profiling reveals three proteins associated to amyotrophic lateral sclerosis.
Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria.
Häggmark A, Mikus M, Mohsenchian A, Hong MG, Forsström B, Gajewska B, Barańczyk-Kuźma A, Uhlén M, Schwenk JM, Kuźma-Kozakiewicz M, Nilsson P
Annals of clinical and translational neurology 2014 Aug;1(8):544-53
Annals of clinical and translational neurology 2014 Aug;1(8):544-53
Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria.
Bachmann J, Burté F, Pramana S, Conte I, Brown BJ, Orimadegun AE, Ajetunmobi WA, Afolabi NK, Akinkunmi F, Omokhodion S, Akinbami FO, Shokunbi WA, Kampf C, Pawitan Y, Uhlén M, Sodeinde O, Schwenk JM, Wahlgren M, Fernandez-Reyes D, Nilsson P
PLoS pathogens 2014 Apr;10(4):e1004038
PLoS pathogens 2014 Apr;10(4):e1004038
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NF-M Antibody [NBP1-87754] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEK 293
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NF-M Antibody [NBP1-87754] - Analysis NBP1-87754 (A) shows similar pattern to independent antibody NBP1-87753 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NF-M Antibody [NBP1-87754] - Analysis in human cell line HEK 293 and human cell line A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebellum shows strong cytoplasmic positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebral cortex shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Analysis in human cerebral cortex and liver tissues. Corresponding NF-M RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebellum shows strong cytoplasmic positivity in white matter.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human liver shows no positivity in hepatocytes as expected.