16480-1-AP

antibody from Proteintech Group

Targeting: NDUFA4L2 COXFA4L2, FLJ26118, NUOMS

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16480-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16480-1-AP, RRID:AB_2150637

Product name

NDUFA4L2 antibody

Antibody type

Polyclonal

Description

KD/KO validated NDUFA4L2 antibody (Cat. #16480-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Novel Ultrastructural Insights into the Clear-Cell Carcinoma of the Pancreas: A Case Report.

Giansante V, Di Angelo L, Calabrese C, De Sanctis P, Regi P, Martelli FM, Stati G, Lattanzio R, Alberti S, Guerra E, Di Pietro R
International journal of molecular sciences 2024 Apr 13;25(8)

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Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma.

Jia Y, Zhang B, Zhang C, Kwong DL, Chang Z, Li S, Wang Z, Han H, Li J, Zhong Y, Sui X, Fu L, Guan X, Qin Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Mar;10(7):e2204565

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Engineered probiotics limit CNS autoimmunity by stabilizing HIF-1α in dendritic cells.

Sanmarco LM, Rone JM, Polonio CM, Giovannoni F, Lahore GF, Ferrara K, Gutierrez-Vazquez C, Li N, Sokolovska A, Plasencia A, Akl CF, Nanda P, Heck ES, Li Z, Lee HG, Chao CC, Rejano-Gordillo CM, Fonseca-Castro PH, Illouz T, Linnerbauer M, Kenison JE, Barilla RM, Farrenkopf D, Piester G, Dailey L, Kuchroo VK, Hava D, Wheeler MA, Clish C, Nowarski R, Balsa E, Lora JM, Quintana FJ
bioRxiv : the preprint server for biology 2023 Mar 21;

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NDUFA4L2 reduces mitochondrial respiration resulting in defective lysosomal trafficking in clear cell renal cell carcinoma.

Kubala JM, Laursen KB, Schreiner R, Williams RM, van der Mijn JC, Crowley MJ, Mongan NP, Nanus DM, Heller DA, Gudas LJ
Cancer biology & therapy 2023 Dec 31;24(1):2170669

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Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells.

Sanmarco LM, Rone JM, Polonio CM, Fernandez Lahore G, Giovannoni F, Ferrara K, Gutierrez-Vazquez C, Li N, Sokolovska A, Plasencia A, Faust Akl C, Nanda P, Heck ES, Li Z, Lee HG, Chao CC, Rejano-Gordillo CM, Fonseca-Castro PH, Illouz T, Linnerbauer M, Kenison JE, Barilla RM, Farrenkopf D, Stevens NA, Piester G, Chung EN, Dailey L, Kuchroo VK, Hava D, Wheeler MA, Clish C, Nowarski R, Balsa E, Lora JM, Quintana FJ
Nature 2023 Aug;620(7975):881-889

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Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy.

Zhong Y, Wang L, Jin R, Liu J, Luo R, Zhang Y, Zhu L, Peng X
Nutrients 2023 Apr 30;15(9)

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MUC1 Tissue Expression and Its Soluble Form CA15-3 Identify a Clear Cell Renal Cell Carcinoma with Distinct Metabolic Profile and Poor Clinical Outcome.

Lucarelli G, Rutigliano M, Loizzo D, di Meo NA, Lasorsa F, Mastropasqua M, Maiorano E, Bizzoca C, Vincenti L, Battaglia M, Ditonno P
International journal of molecular sciences 2022 Nov 12;23(22)

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NDUFA4 promotes cell proliferation by enhancing oxidative phosphorylation in pancreatic adenocarcinoma.

Zhang Y, Ge M, Chen Y, Yang Y, Chen W, Wu D, Cai H, Chen X, Wu X
Journal of bioenergetics and biomembranes 2022 Dec;54(5-6):283-291

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NDUFA4L2 promotes trastuzumab resistance in HER2-positive breast cancer.

Yuan Y, Gao H, Zhuang Y, Wei L, Yu J, Zhang Z, Zhang L, Wang L
Therapeutic advances in medical oncology 2021;13:17588359211027836

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NDUFA4L2 in smooth muscle promotes vascular remodeling in hypoxic pulmonary arterial hypertension.

Liu Y, Nie X, Zhu J, Wang T, Li Y, Wang Q, Sun Z
Journal of cellular and molecular medicine 2021 Jan;25(2):1221-1237

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Mitochondrial NDUFA4L2 is a novel regulator of skeletal muscle mass and force.

Liu Z, Chaillou T, Santos Alves E, Mader T, Jude B, Ferreira DMS, Hynynen H, Cheng AJ, Jonsson WO, Pironti G, Andersson DC, Kenne E, Ruas JL, Tavi P, Lanner JT
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Dec;35(12):e22010

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NDUFA4L2 Regulated by HIF-1α Promotes Metastasis and Epithelial-Mesenchymal Transition of Osteosarcoma Cells Through Inhibiting ROS Production.

Xu WN, Yang RZ, Zheng HL, Jiang LS, Jiang SD
Frontiers in cell and developmental biology 2020;8:515051

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Acute O(2) sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.

Moreno-Domínguez A, Ortega-Sáenz P, Gao L, Colinas O, García-Flores P, Bonilla-Henao V, Aragonés J, Hüttemann M, Grossman LI, Weissmann N, Sommer N, López-Barneo J
Science signaling 2020 Jan 21;13(615)

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Mitochondrial NDUFA4L2 attenuates the apoptosis of nucleus pulposus cells induced by oxidative stress via the inhibition of mitophagy.

Xu WN, Zheng HL, Yang RZ, Liu T, Yu W, Zheng XF, Li B, Jiang SD, Jiang LS
Experimental & molecular medicine 2019 Nov 18;51(11):1-16

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Mitochondrial NDUFA4L2 protein promotes the vitality of lung cancer cells by repressing oxidative stress.

Meng L, Yang X, Xie X, Wang M
Thoracic cancer 2019 Apr;10(4):676-685

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Fatty acid binding protein 7 may be a marker and therapeutic targets in clear cell renal cell carcinoma.

Nagao K, Shinohara N, Smit F, de Weijert M, Jannink S, Owada Y, Mulders P, Oosterwijk E, Matsuyama H
BMC cancer 2018 Nov 15;18(1):1114

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Integrated multi-omics characterization reveals a distinctive metabolic signature and the role of NDUFA4L2 in promoting angiogenesis, chemoresistance, and mitochondrial dysfunction in clear cell renal cell carcinoma.

Lucarelli G, Rutigliano M, Sallustio F, Ribatti D, Giglio A, Lepore Signorile M, Grossi V, Sanese P, Napoli A, Maiorano E, Bianchi C, Perego RA, Ferro M, Ranieri E, Serino G, Bell LN, Ditonno P, Simone C, Battaglia M
Aging 2018 Dec 11;10(12):3957-3985

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Gene expression analyses reveal metabolic specifications in acute O(2) -sensing chemoreceptor cells.

Gao L, Bonilla-Henao V, García-Flores P, Arias-Mayenco I, Ortega-Sáenz P, López-Barneo J
The Journal of physiology 2017 Sep 15;595(18):6091-6120

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Single cell transcriptome analysis of mouse carotid body glomus cells.

Zhou T, Chien MS, Kaleem S, Matsunami H
The Journal of physiology 2016 Aug 1;594(15):4225-51

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Metabolic adaptation to chronic hypoxia in cardiac mitochondria.

Heather LC, Cole MA, Tan JJ, Ambrose LJ, Pope S, Abd-Jamil AH, Carter EE, Dodd MS, Yeoh KK, Schofield CJ, Clarke K
Basic research in cardiology 2012 May;107(3):268

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Thyroid paraganglioma. Report of 3 cases and description of an immunohistochemical profile useful in the differential diagnosis with medullary thyroid carcinoma, based on complementary DNA array results.

Castelblanco E, Gallel P, Ros S, Gatius S, Valls J, De-Cubas AA, Maliszewska A, Yebra-Pimentel MT, Menarguez J, Gamallo C, Opocher G, Robledo M, Matias-Guiu X
Human pathology 2012 Jul;43(7):1103-12

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Induction of the mitochondrial NDUFA4L2 protein by HIF-1α decreases oxygen consumption by inhibiting Complex I activity.

Tello D, Balsa E, Acosta-Iborra B, Fuertes-Yebra E, Elorza A, Ordóñez Á, Corral-Escariz M, Soro I, López-Bernardo E, Perales-Clemente E, Martínez-Ruiz A, Enríquez JA, Aragonés J, Cadenas S, Landázuri MO
Cell metabolism 2011 Dec 7;14(6):768-79

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16480-1-AP(NDUFA4L2 antibody) at dilution of 1:600

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using NDUFA4L2 antibody 16480-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 16480-1-AP(NDUFA4L2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 16480-1-AP(NDUFA4L2 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue