- 720257 - Invitrogen Antibodies TEX10 antibody

Liu Y, Xu G, Li L
Oncology reports 2021 May;45(5)

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence was performed on fixed and permeabilized U-87 MG cells for detection of endogenous TEX10 using anti-TEX10 Rabbit Polyclonal Antibody (Product # 720257, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TEX10 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TEX10. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence was performed on fixed and permeabilized U-87 MG cells for detection of endogenous TEX10 using anti-TEX10 Rabbit Polyclonal Antibody (Product # 720257, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TEX10 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TEX10. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow Cytometry analysis of endogenous Tex10 was performed on K-562 cells labeled with Anti-Tex10 Rabbit Polyclonal Antibody (Product# 720257, 5 ug/ 1M cells) or with Rabbit isotype control and detected with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, (Alexa Fluor® 488 conjugate, Product# A27034, 0.4 ug/ml, 1:2500) as represented by the red and pink histograms respectively. The purple histogram represents unstained control cells and the green histogram represents no-primary-Antibody control. A representative of 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer (4468770).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4. miR-30b-5p directly targets the 3''-UTR of Tex10 to suppress its expression. (A) The mRNA expression of Tex10 in 30 pairs of PC tissues and matched normal tissues was assessed by qPCR. *P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. miR-30b-5p-Tex10 axis partially mediates the effects of GATA3-AS1. AsPC-1 cells were transfected with pcDNA 3.1 empty plasmids, pcDNA 3.1-GATA3-AS1 plasmids, a combination of pcDNA 3.1-GATA3-AS1 plasmids and miR-30b-5p mimic, or a combination of pcDNA 3.1-GATA3-AS1 plasmids, miR-30b-5p mimic, and pcDNA3.1-Tex10 plasmids. (A) The protein expression of Tex10 was assessed by western blotting. (B) Cell viability was assessed by Cell Counting Kit-8 assay at 0, 24, 48, 72 and 96 h post transfection. (C) Cell proliferation was assessed by colony formation assay. (D) Cell apoptosis was assessed by flow cytometry. (E) Cell invasion was assessed by Transwell assay. (F) The stemness was assessed by spheroid formation assay. The protein expression of (G) Wnt1 and (H) beta-catenin was assessed by western blotting. *P

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