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- References [10]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 15624-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15624-1-AP, RRID:AB_2211330
- Product name
- U2AF65 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated U2AF65 antibody (Cat. #15624-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Exosomal U2AF2 derived from human bone marrow mesenchymal stem cells attenuates the intervertebral disc degeneration through circ_0036763/miR-583/ACAN axis.
A splicing silencer in SMN2 intron 6 is critical in spinal muscular atrophy.
Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.
RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m(6)A-dependent manner.
U2 small nuclear RNA auxiliary factor 2, transcriptionally activated by the transcription factor Dp-1/E2F transcription factor 1 complex, enhances the growth and aerobic glycolysis of leiomyosarcoma cells.
Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.
Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.
In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.
U2AF65 enhances milk synthesis and growth of bovine mammary epithelial cells by positively regulating the mTOR-SREBP-1c signalling pathway.
Identification of novel PANDAR protein interaction partners involved in splicing regulation.
Chen X, Cai D, Li H, Wei Q, Li X, Han Z, Liang J, Xie J, Ruan J, Liu J, Xiang Z, Dong W, Guo W
Regenerative therapy 2024 Mar;25:344-354
Regenerative therapy 2024 Mar;25:344-354
A splicing silencer in SMN2 intron 6 is critical in spinal muscular atrophy.
Wang L, Ji Y, Chen Y, Bai J, Gao P, Feng P
Human molecular genetics 2023 Mar 6;32(6):971-983
Human molecular genetics 2023 Mar 6;32(6):971-983
Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.
Iwasaki M, Kawahara Y, Okubo C, Yamakawa T, Nakamura M, Tabata T, Nishi Y, Narita M, Ohta A, Saito H, Yamamoto T, Nakagawa M, Yamanaka S, Takahashi K
iScience 2022 May 20;25(5):104289
iScience 2022 May 20;25(5):104289
RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m(6)A-dependent manner.
Sun S, Gao T, Pang B, Su X, Guo C, Zhang R, Pang Q
Cell death & disease 2022 Jan 21;13(1):73
Cell death & disease 2022 Jan 21;13(1):73
U2 small nuclear RNA auxiliary factor 2, transcriptionally activated by the transcription factor Dp-1/E2F transcription factor 1 complex, enhances the growth and aerobic glycolysis of leiomyosarcoma cells.
Li Y, Chen S, Zhang X, Zhuo N
Bioengineered 2022 Apr;13(4):10200-10212
Bioengineered 2022 Apr;13(4):10200-10212
Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.
Fang L, Ye T, An Y
Journal of immunology research 2021;2021:8050993
Journal of immunology research 2021;2021:8050993
Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.
Gong X, Tian M, Cao N, Yang P, Xu Z, Zheng S, Liao Q, Chen C, Zeng C, Jose PA, Wang DZ, Jian Z, Xiao Y, Jiang DS, Wei X, Zhang B, Wang Y, Chen K, Wu G, Zeng C
The Journal of clinical investigation 2021 Dec 15;131(24)
The Journal of clinical investigation 2021 Dec 15;131(24)
In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.
Sun L, Li P, Ju X, Rao J, Huang W, Ren L, Zhang S, Xiong T, Xu K, Zhou X, Gong M, Miska E, Ding Q, Wang J, Zhang QC
Cell 2021 Apr 1;184(7):1865-1883.e20
Cell 2021 Apr 1;184(7):1865-1883.e20
U2AF65 enhances milk synthesis and growth of bovine mammary epithelial cells by positively regulating the mTOR-SREBP-1c signalling pathway.
Yu Y, Zhen Z, Qi H, Yuan X, Gao X, Zhang M
Cell biochemistry and function 2019 Mar;37(2):93-101
Cell biochemistry and function 2019 Mar;37(2):93-101
Identification of novel PANDAR protein interaction partners involved in splicing regulation.
Pospiech N, Cibis H, Dietrich L, Müller F, Bange T, Hennig S
Scientific reports 2018 Feb 12;8(1):2798
Scientific reports 2018 Feb 12;8(1):2798
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 15624-1-AP(U2AF2 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The U2AF65 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human U2AF65. This antibody recognizes human,mouse,rat antigen. The U2AF65 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 15624-1-AP(U2AF2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
