Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-95999 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- U2AF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NQDKNFAFLE FRSVDETTQA MAFDGIIFQG QSLKIRRPHD YQPLPGMSEN PSVYVPGVVS TVVPDSAHKL FIGGLPNYLN DDQVKELLTS FGPLKAFNLV KDSATGLSKG YAFCEYVDIN VTDQAIAGLN GMQLGDKKLL VQRASVGAKN ATLSTINQTP VTLQVPGLMS SQVQMGGHPT EVLCLMNMVL PEELLDDEEY EEIVEDVRDE CSKYGLVKSI EIPRPVDGVE VPGCGKIFVE FTSVFDCQKA MQGLTGRKFA NRVVVTKYCD PDSYHRRDFW; Positive Samples: Jurkat, SW620, Mouse spleen; Cellular Location: Nucleus
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using U2AF2 Polyclonal antibody (Product # PA5-95999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of U2AF2 in extracts of various cell lines using U2AF2 Polyclonal Antibody (Product # PA5-95999) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), A-431 (Lane 3), and Jurkat (Lane 4). The blot was probed with Anti-U2AF2 Polyclonal Antibody (Product # PA5-95999, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 55 kDa band corresponding to U2AF2 was detected in cell lines and tissue tested along with uncharacterized bands at ~42 kDa and ~82 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of U2AF2 in extracts of various cell lines using U2AF2 Polyclonal Antibody (Product # PA5-95999) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of U2AF2 was performed in U2OS cells using U2AF2 Polyclonal Antibody (Product # PA5-95999).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of U2AF2 was performed in 200 µg extracts of SW620 cells using U2AF2 Polyclonal Antibody (Product # PA5-95999). Western blot was performed from the immunoprecipitate using U2AF2 Polyclonal Antibody.