Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- EB10018 - Provider product page
- Provider
- Everest Biotech
- Product name
- Goat anti-POU2AF1 Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-POU2AF1 Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-DSDAYALNHTLSVE
- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with EB10018(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB10018(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5µg/ml.