Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA807930 - Provider product page
- Provider
- OriGene
- Product name
- POU2AF1 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)
- Antibody type
- Monoclonal
- Description
- POU2AF1 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- POU2AF1
- Isotype
- IgG
- Antibody clone number
- OTI2D3
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807930)(1:150)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120å¡C for 3 min, TA807930)(1:150)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-POU2AF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA807930)(1:200)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of penetrated Ramos cells, using anti-POU2AF1 antibody(TA807930),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue). The right is Jurkat cells as negtive control.(1:100)
- Validation comment
- FC
