Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB19692 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB19692, RRID:AB_10903805
- Product name
- POU2AF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of POU2AF1.
- Antigen sequence
C-DSDAYALNHTLSVE
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human POU2AF1 with DYKDDDDK tag probed with POU2AF1 polyclonal antibody (Cat # PAB19692) (1 ug/mL) in lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with POU2AF1 polyclonal antibody (Cat # PAB19692) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- POU2AF1 polyclonal antibody (Cat # PAB19692) (3-5 ug/mL) staining of paraffin embedded human spleen, shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)