Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP32080PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti POU2AF1 / BOB1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the C-Terminus of Human POU2AF1 (NP_006226.2)
- Reactivity
- Human, Bovine, Canine
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with POU2AF1 Antibody Cat.-No AP32080PU-N (1 µg/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/3000) in Lane C. Mock-transfected HEK293 probed with POU2AF1 Antibody AP32080PU-N (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Paraffin Embedded Human Spleen using POU2AF1 / BOB1 Antibody Cat.-No AP32080PU-N shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells.