AF3540

antibody from Novus Biologicals

Targeting: GFI1 GFI-1, GFI1A, ZNF163

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3540

Provider

Novus Biologicals

Product name

Goat Polyclonal GFI-1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human GFI-1 in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Transcription factor induced conversion of human fibroblasts towards the hair cell lineage.

Duran Alonso MB, Lopez Hernandez I, de la Fuente MA, Garcia-Sancho J, Giraldez F, Schimmang T
PloS one 2018;13(7):e0200210

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GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1.

Vadnais C, Chen R, Fraszczak J, Yu Z, Boulais J, Pinder J, Frank D, Khandanpour C, Hébert J, Dellaire G, Côté JF, Richard S, Orthwein A, Drobetsky E, Möröy T
Nature communications 2018 Apr 12;9(1):1418

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Threshold Levels of Gfi1 Maintain E2A Activity for B Cell Commitment via Repression of Id1.

Fraszczak J, Helness A, Chen R, Vadnais C, Robert F, Khandanpour C, Möröy T
PloS one 2016;11(7):e0160344

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Single-cell analysis of mixed-lineage states leading to a binary cell fate choice.

Olsson A, Venkatasubramanian M, Chaudhri VK, Aronow BJ, Salomonis N, Singh H, Grimes HL
Nature 2016 Sep 29;537(7622):698-702

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of GFI-1 in THP-1 Human Cell Line by Flow Cytometry. Detection of GFI-1 in THP-1 Human Cell Line by Flow Cytometry.