Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 710851 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p19 INK4d Recombinant Polyclonal Antibody (14HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 14HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK- 293 (Lane1), HEK- 293 treated with Trichostatin A, (Lane 2), HeLa (Lane 3), HeLa treated with Trichostatin A (Lane 4), HCT 116 (Lane 5), HCT 116 treated with Trichostatin A (Lane 6) (2uM/24hrs). The blots were probed with Anti-P19INK4D Recombinant Rabbit Polyclonal Antibody (Product # 710851, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 18 kDa band corresponding to P19INK4D was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- P19INK4D was immunoprecipitated using 2 µg of the P19INK4D Recombinant Rabbit Polyclonal Antibody (Product # 710851) from lysate of HeLa cells treated with Trichostatin A (2 uM/24 hours) (Lane 3) using the Dynabeads® Protein A Immunoprecipitation Kit (Product # 10006D).Normal Rabbit IgG was used as a negative IP control (Lane 2). 10% input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using P19INK4D Recombinant Rabbit Polyclonal Antibody (Product # 710851) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).