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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00001445-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001445-M01, RRID:AB_538031
- Product name
- Mouse Monoclonal CSK Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CSK - c-src tyrosine kinase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Targeted Deep Sequencing in Multiple-Affected Sibships of European Ancestry Identifies Rare Deleterious Variants in PTPN22 That Confer Risk for Type 1 Diabetes.
Ge Y, Onengut-Gumuscu S, Quinlan AR, Mackey AJ, Wright JA, Buckner JH, Habib T, Rich SS, Concannon P
Diabetes 2016 Mar;65(3):794-802
Diabetes 2016 Mar;65(3):794-802
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi ( Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3 Analysis of CSK expression in HL-60.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK transfected lysate using anti-CSK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CSK MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CSK Antibody (3A3) [H00001445-M01] - Detection limit for recombinant GST tagged CSK is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CSK Antibody (3A3) [H00001445-M01] - Analysis of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
