Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-5773 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FXR2 Monoclonal Antibody (A42)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-5773 detects FXR2 in human samples and is predicted to function in mouse samples. MA1-5773 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures. MA1-5773 detects a band of approximately 92 kDa. The MA1-5773 immunogen is His6-FXR2s fusion protein produced in a bacterial pET system.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- A42
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), U-87 MG (Lane 4), PC-3 (Lane 5) and NIH/3T3 (Lane 6). The blot was probed with Anti-FXR2 Monoclonal Antibody (A42) (Product # MA1-5773, 1:1000 dilution) and detected by chemiluminescence using Goat anti Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 95 kDa band corresponding to FXR2 was observed across all cell lines tested.