Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28979 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FXR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2. Predicted reactivity: Mouse (99%), Rat (99%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references FXR1 regulates transcription and is required for growth of human cancer cells with TP53/FXR2 homozygous deletion.
Fan Y, Yue J, Xiao M, Han-Zhang H, Wang YV, Ma C, Deng Z, Li Y, Yu Y, Wang X, Niu S, Hua Y, Weng Z, Atadja P, Li E, Xiang B
eLife 2017 Aug 2;6
eLife 2017 Aug 2;6
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FXR2 Polyclonal Antibody (Product # PA5-28979). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2 . 7.5% SDS PAGE. FXR2 Polyclonal Antibody (Product # PA5-28979) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FXR2 (Product # PA5-28979) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. FXR1 regulates transcription by colocalizing with H3K4me3 and STAT1 and STAT3 at gene promoters. ( A ) Genomic distribution of FXR1 ChIP-seq peaks in H358 cells. 22% of peaks are enriched at promoter regions (defined as 5000 bp upstream and 1000 bp downstream of TSS) (p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5--figure supplement 4. FXR1 colocalizes with FXR2, STAT1/3 and H3K4me3 at gene promoters in AGS cells. ( A ) Genomic distribution of FXR1, FXR2, H3K4me3, STAT1 and STAT3 ChIP-seq peaks in AGS cells. ( B ) Venn diagram showing significant overlap of FXR1 and FXR2 (upper), FXR1, STAT1, STAT3, and H3K4me3 (lower) ChIP-seq peak(enriched at TSS)-associated genes in AGS cells (p