PA5-24816

antibody from Invitrogen Antibodies

Targeting: COQ9 C16orf49, DKFZP434K046

Western blot

Immunohistochemistry

Antibody data

Submit

Validation data

Product number: PA5-24816 - Provider product page
Provider: Invitrogen Antibodies
Product name: COQ9 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with rat based on sequence homology.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 400 µL
Concentration: 0.49 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

COQ9 protein structure - PA5-24816 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Levels of COQ biosynthetic proteins A-D Representative Western blot and quantitation of Western blot bands of COQ7 (A), ADCK3 (B), COQ5 (C) and COQ6 (D), and VDAC1 as a loading control in the kidneys of 3-month-old mice. * P < 0.05; ** P < 0.01; *** P < 0.001; Coq9 Q95X and Coq9 R239X mice versus Coq9 +/+ mice. ## P < 0.01; ### P < 0.001; Coq9 Q95X versus Coq9 R239X mice. One-way ANOVA with a Tukey's post hoc test. E-H Representative Western blot and quantitation of Western blot bands of COQ7 (E), ADCK3 (F), COQ5 (G) and COQ6 (H), and VDAC1 as a loading control in skeletal muscle of 3-month-old mice. ** P < 0.01; *** P < 0.001; Coq9 Q95X and Coq9 R239X mice versus Coq9 +/+ mice. # P < 0.05; ## P < 0.01; Coq9 Q95X versus Coq9 R239X mice. Data information: All values are presented as mean +- SD. One-way ANOVA with a Tukey's post hoc test. Numbers above columns indicate P -values of the one-way ANOVA test. Coq9 +/+ mice n = 4; Coq9 Q95X and Coq9 R239X mice n = 5. Source data are available online for this figure.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Moderate CoQ deficiency in Coq9 Q95X mice leads to impaired mitochondrial bioenergetics function A-C Mitochondrial CoQ 9 levels from cerebrum (A), kidney (B) and skeletal muscle (C) of Coq9 +/+ and Coq9 Q95X males and females. n = 8 for each group. D-F CI+CIII activity in cerebrum (D), kidney (E) and skeletal muscle (F) of male and female Coq9 +/+ and Coq9 Q95X mice. n = 6 for each group. G-I CII+CIII activity in cerebrum (G), kidney (H) and skeletal muscle (I) of male and female Coq9 +/+ and Coq9 Q95X mice. n = 6 for each group. J-L Blue-native gel electrophoresis (BNGE) followed by immunoblotting analysis of mitochondrial supercomplexes from Coq9 +/+ ( n = 3) and Coq9 Q95X mice ( n = 4) at 3 months of age. Data information: (A-I) Data are expressed as mean +- SD. Statistical analyses were performed on Coq9 +/+ male mice versus Coq9 Q95X male mice and Coq9 +/+ female mice versus Coq9 Q95X female mice. ** P < 0.01; *** P < 0.001. Student's t -test. Complex I+III, NADH-cytochrome c reductase; complex II+III, SDH-cytochrome c reductase. Source data are available online for this figure.

Supportive validation