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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324451 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal DDIT3 Antibody (C-term A135)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal DDIT3 Antibody (C-term A135)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- DDIT3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- DDIT3 Antibody (C-term A135) (Cat. #TA324451) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with DDIT3 Antibody (C-term A135)(Cat#TA324451/SA101207AA).Hela cells were incubated with DDIT3 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). DDIT3 immunoreactivity is localized to Cytoplasm significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- DDIT3 Antibody (C-term A135) (Cat. #TA324451) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
