BS-5177R

antibody from Invitrogen Antibodies

Targeting: DDIT3 CHOP, CHOP10, GADD153

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

BS-5177R

Provider

Invitrogen Antibodies

Product name

Phospho-GADD153 (Ser30) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-phospho-GADD153 (Ser30) Polyclonal Antibody, Unconjugated (bs-5177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GADD153 (Ser30) Polyclonal Antibody, Unconjugated (bs-5177R) at 1:400 overnight at 4°C, DAB staining.