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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-28956 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHOP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HeLa, HeLa treated tunicaycin 8 hr.
- Concentration
- 1 mg/mL
Submitted references Schizandrin A induces the apoptosis and suppresses the proliferation, invasion and migration of gastric cancer cells by activating endoplasmic reticulum stress.
Pu H, Qian Q, Wang F, Gong M, Ge X
Molecular medicine reports 2021 Nov;24(5)
Molecular medicine reports 2021 Nov;24(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GADD153 using 30 µg of A) HeLa and B) HeLa treated with tunicamycin (8 hr) lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a GADD153 polyclonal antibody (Product # PA5-28956) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CHOP Polyclonal Antibody (Product # PA5-28956). Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHOP Polyclonal Antibody (Product # PA5-28956) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CHOP Polyclonal Antibody (Product # PA5-28956) and a 21kDa band corresponding to CHOP was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of K-562 (Lane 1), HeLa (Lane 2), HeLa treated with Tunicamycin (Lane 3), A549 (Lane 4), A549 treated with Thapsigargin (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:2000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CHOP was performed in A549 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CHOP Polyclonal Antibody (Product # PA5-28956) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
