NB600-1335
antibody from Novus Biologicals
Targeting: DDIT3
CHOP, CHOP10, GADD153
Antibody data
- Antibody Data
- Antigen structure
- References [35]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-1335 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1335, RRID:AB_2089251
- Product name
- Mouse Monoclonal GADD153/CHOP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Antigen sequence
The N-terminal region of CHOP/GADD1
53.- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Deficiency of the ER-stress-regulator MANF triggers progressive outer hair cell death and hearing loss.
FET family fusion oncoproteins target the SWI/SNF chromatin remodeling complex.
Cannabidiol Enhances the Therapeutic Effects of TRAIL by Upregulating DR5 in Colorectal Cancer.
Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresponsiveness by inhibiting UPR transducers.
Short-term high-fat diet modulates several inflammatory, ER stress, and apoptosis markers in the hippocampus of young mice.
A FBN1 3'UTR mutation variant is associated with endoplasmic reticulum stress in aortic aneurysm in Marfan syndrome.
A Novel Animal Model for Studying Depression Featuring the Induction of the Unfolded Protein Response in Hippocampus.
Gingival Periodontal Disease (PD) Level-Butyric Acid Affects the Systemic Blood and Brain Organ: Insights Into the Systemic Inflammation of Periodontal Disease.
Placental hypoxia inducible factor -1α & CHOP immuno-histochemical expression relative to maternal circulatory syncytiotrophoblast micro-vesicles in preeclamptic and normotensive pregnancies.
Piperine ameliorates SCA17 neuropathology by reducing ER stress.
HIF-1α triggers ER stress and CHOP-mediated apoptosis in alveolar epithelial cells, a key event in pulmonary fibrosis.
Impaired autophagic activity and ATG4B deficiency are associated with increased endoplasmic reticulum stress-induced lung injury.
CRISPR-Cas9-based treatment of myocilin-associated glaucoma.
Hepatic overproduction of 13-HODE due to ALOX15 upregulation contributes to alcohol-induced liver injury in mice.
Endoplasmic reticulum stress-induced apoptosis accompanies enhanced expression of multiple inositol polyphosphate phosphatase 1 (Minpp1): a possible role for Minpp1 in cellular stress response.
Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway.
Zinc deficiency mediates alcohol-induced apoptotic cell death in the liver of rats through activating ER and mitochondrial cell death pathways.
CYP46A1 inhibition, brain cholesterol accumulation and neurodegeneration pave the way for Alzheimer's disease.
The autophagy-related marker LC3 can predict prognosis in human hepatocellular carcinoma.
Inhibition of mitochondria- and endoplasmic reticulum stress-mediated autophagy augments temozolomide-induced apoptosis in glioma cells.
Amyloid formation in human IAPP transgenic mouse islets and pancreas, and human pancreas, is not associated with endoplasmic reticulum stress.
CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI.
CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI.
Induction of a secreted protein by the myxoid liposarcoma oncogene.
Induction of a secreted protein by the myxoid liposarcoma oncogene.
Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
Identification of novel stress-induced genes downstream of chop.
Regulation of the pro-B-cell-specific enhancer of the Id1 gene involves the C/EBP family of proteins.
Regulation of the pro-B-cell-specific enhancer of the Id1 gene involves the C/EBP family of proteins.
TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling.
Stress-induced phosphorylation and activation of the transcription factor CHOP (GADD153) by p38 MAP Kinase.
Stress-induced phosphorylation and activation of the transcription factor CHOP (GADD153) by p38 MAP Kinase.
Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153).
Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153).
Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153).
Herranen A, Ikäheimo K, Lankinen T, Pakarinen E, Fritzsch B, Saarma M, Lindahl M, Pirvola U
Cell death & disease 2020 Feb 6;11(2):100
Cell death & disease 2020 Feb 6;11(2):100
FET family fusion oncoproteins target the SWI/SNF chromatin remodeling complex.
Lindén M, Thomsen C, Grundevik P, Jonasson E, Andersson D, Runnberg R, Dolatabadi S, Vannas C, Luna Santamarίa M, Fagman H, Ståhlberg A, Åman P
EMBO reports 2019 May;20(5)
EMBO reports 2019 May;20(5)
Cannabidiol Enhances the Therapeutic Effects of TRAIL by Upregulating DR5 in Colorectal Cancer.
Kim JL, Kim BR, Kim DY, Jeong YA, Jeong S, Na YJ, Park SH, Yun HK, Jo MJ, Kim BG, Kim HD, Kim DH, Oh SC, Lee SI, Lee DH
Cancers 2019 May 9;11(5)
Cancers 2019 May 9;11(5)
Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresponsiveness by inhibiting UPR transducers.
Nakada EM, Bhakta NR, Korwin-Mihavics BR, Kumar A, Chamberlain N, Bruno SR, Chapman DG, Hoffman SM, Daphtary N, Aliyeva M, Irvin CG, Dixon AE, Woodruff PG, Amin S, Poynter ME, Desai DH, Anathy V
JCI insight 2019 May 2;4(9)
JCI insight 2019 May 2;4(9)
Short-term high-fat diet modulates several inflammatory, ER stress, and apoptosis markers in the hippocampus of young mice.
Nakandakari SCBR, Muñoz VR, Kuga GK, Gaspar RC, Sant'Ana MR, Pavan ICB, da Silva LGS, Morelli AP, Simabuco FM, da Silva ASR, de Moura LP, Ropelle ER, Cintra DE, Pauli JR
Brain, behavior, and immunity 2019 Jul;79:284-293
Brain, behavior, and immunity 2019 Jul;79:284-293
A FBN1 3'UTR mutation variant is associated with endoplasmic reticulum stress in aortic aneurysm in Marfan syndrome.
Siegert AM, García Díaz-Barriga G, Esteve-Codina A, Navas-Madroñal M, Gorbenko Del Blanco D, Alberch J, Heath S, Galán M, Egea G
Biochimica et biophysica acta. Molecular basis of disease 2019 Jan;1865(1):107-114
Biochimica et biophysica acta. Molecular basis of disease 2019 Jan;1865(1):107-114
A Novel Animal Model for Studying Depression Featuring the Induction of the Unfolded Protein Response in Hippocampus.
Timberlake Ii M, Roy B, Dwivedi Y
Molecular neurobiology 2019 Dec;56(12):8524-8536
Molecular neurobiology 2019 Dec;56(12):8524-8536
Gingival Periodontal Disease (PD) Level-Butyric Acid Affects the Systemic Blood and Brain Organ: Insights Into the Systemic Inflammation of Periodontal Disease.
Cueno ME, Ochiai K
Frontiers in immunology 2018;9:1158
Frontiers in immunology 2018;9:1158
Placental hypoxia inducible factor -1α & CHOP immuno-histochemical expression relative to maternal circulatory syncytiotrophoblast micro-vesicles in preeclamptic and normotensive pregnancies.
Verma S, Pillay P, Naicker T, Moodley J, Mackraj I
European journal of obstetrics, gynecology, and reproductive biology 2018 Jan;220:18-24
European journal of obstetrics, gynecology, and reproductive biology 2018 Jan;220:18-24
Piperine ameliorates SCA17 neuropathology by reducing ER stress.
Guo J, Cui Y, Liu Q, Yang Y, Li Y, Weng L, Tang B, Jin P, Li XJ, Yang S, Li S
Molecular neurodegeneration 2018 Jan 30;13(1):4
Molecular neurodegeneration 2018 Jan 30;13(1):4
HIF-1α triggers ER stress and CHOP-mediated apoptosis in alveolar epithelial cells, a key event in pulmonary fibrosis.
Delbrel E, Soumare A, Naguez A, Label R, Bernard O, Bruhat A, Fafournoux P, Tremblais G, Marchant D, Gille T, Bernaudin JF, Callard P, Kambouchner M, Martinod E, Valeyre D, Uzunhan Y, Planès C, Boncoeur E
Scientific reports 2018 Dec 18;8(1):17939
Scientific reports 2018 Dec 18;8(1):17939
Impaired autophagic activity and ATG4B deficiency are associated with increased endoplasmic reticulum stress-induced lung injury.
Maciel M, Hernández-Barrientos D, Herrera I, Selman M, Pardo A, Cabrera S
Aging 2018 Aug 27;10(8):2098-2112
Aging 2018 Aug 27;10(8):2098-2112
CRISPR-Cas9-based treatment of myocilin-associated glaucoma.
Jain A, Zode G, Kasetti RB, Ran FA, Yan W, Sharma TP, Bugge K, Searby CC, Fingert JH, Zhang F, Clark AF, Sheffield VC
Proceedings of the National Academy of Sciences of the United States of America 2017 Oct 17;114(42):11199-11204
Proceedings of the National Academy of Sciences of the United States of America 2017 Oct 17;114(42):11199-11204
Hepatic overproduction of 13-HODE due to ALOX15 upregulation contributes to alcohol-induced liver injury in mice.
Zhang W, Zhong W, Sun Q, Sun X, Zhou Z
Scientific reports 2017 Aug 21;7(1):8976
Scientific reports 2017 Aug 21;7(1):8976
Endoplasmic reticulum stress-induced apoptosis accompanies enhanced expression of multiple inositol polyphosphate phosphatase 1 (Minpp1): a possible role for Minpp1 in cellular stress response.
Kilaparty SP, Agarwal R, Singh P, Kannan K, Ali N
Cell stress & chaperones 2016 Jul;21(4):593-608
Cell stress & chaperones 2016 Jul;21(4):593-608
Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway.
Ying R, Wang XQ, Yang Y, Gu ZJ, Mai JT, Qiu Q, Chen YX, Wang JF
Life sciences 2016 Jan 1;144:208-17
Life sciences 2016 Jan 1;144:208-17
Zinc deficiency mediates alcohol-induced apoptotic cell death in the liver of rats through activating ER and mitochondrial cell death pathways.
Sun Q, Zhong W, Zhang W, Li Q, Sun X, Tan X, Sun X, Dong D, Zhou Z
American journal of physiology. Gastrointestinal and liver physiology 2015 May 1;308(9):G757-66
American journal of physiology. Gastrointestinal and liver physiology 2015 May 1;308(9):G757-66
CYP46A1 inhibition, brain cholesterol accumulation and neurodegeneration pave the way for Alzheimer's disease.
Djelti F, Braudeau J, Hudry E, Dhenain M, Varin J, Bièche I, Marquer C, Chali F, Ayciriex S, Auzeil N, Alves S, Langui D, Potier MC, Laprevote O, Vidaud M, Duyckaerts C, Miles R, Aubourg P, Cartier N
Brain : a journal of neurology 2015 Aug;138(Pt 8):2383-98
Brain : a journal of neurology 2015 Aug;138(Pt 8):2383-98
The autophagy-related marker LC3 can predict prognosis in human hepatocellular carcinoma.
Lee YJ, Hah YJ, Kang YN, Kang KJ, Hwang JS, Chung WJ, Cho KB, Park KS, Kim ES, Seo HY, Kim MK, Park KG, Jang BK
PloS one 2013;8(11):e81540
PloS one 2013;8(11):e81540
Inhibition of mitochondria- and endoplasmic reticulum stress-mediated autophagy augments temozolomide-induced apoptosis in glioma cells.
Lin CJ, Lee CC, Shih YL, Lin CH, Wang SH, Chen TH, Shih CM
PloS one 2012;7(6):e38706
PloS one 2012;7(6):e38706
Amyloid formation in human IAPP transgenic mouse islets and pancreas, and human pancreas, is not associated with endoplasmic reticulum stress.
Hull RL, Zraika S, Udayasankar J, Aston-Mourney K, Subramanian SL, Kahn SE
Diabetologia 2009 Jun;52(6):1102-11
Diabetologia 2009 Jun;52(6):1102-11
CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI.
Sok J, Wang XZ, Batchvarova N, Kuroda M, Harding H, Ron D
Molecular and cellular biology 1999 Jan;19(1):495-504
Molecular and cellular biology 1999 Jan;19(1):495-504
CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI.
Sok J, Wang XZ, Batchvarova N, Kuroda M, Harding H, Ron D
Molecular and cellular biology 1999 Jan;19(1):495-504
Molecular and cellular biology 1999 Jan;19(1):495-504
Induction of a secreted protein by the myxoid liposarcoma oncogene.
Kuroda M, Wang X, Sok J, Yin Y, Chung P, Giannotti JW, Jacobs KA, Fitz LJ, Murtha-Riel P, Turner KJ, Ron D
Proceedings of the National Academy of Sciences of the United States of America 1999 Apr 27;96(9):5025-30
Proceedings of the National Academy of Sciences of the United States of America 1999 Apr 27;96(9):5025-30
Induction of a secreted protein by the myxoid liposarcoma oncogene.
Kuroda M, Wang X, Sok J, Yin Y, Chung P, Giannotti JW, Jacobs KA, Fitz LJ, Murtha-Riel P, Turner KJ, Ron D
Proceedings of the National Academy of Sciences of the United States of America 1999 Apr 27;96(9):5025-30
Proceedings of the National Academy of Sciences of the United States of America 1999 Apr 27;96(9):5025-30
Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
Wang XZ, Harding HP, Zhang Y, Jolicoeur EM, Kuroda M, Ron D
The EMBO journal 1998 Oct 1;17(19):5708-17
The EMBO journal 1998 Oct 1;17(19):5708-17
Identification of novel stress-induced genes downstream of chop.
Wang XZ, Kuroda M, Sok J, Batchvarova N, Kimmel R, Chung P, Zinszner H, Ron D
The EMBO journal 1998 Jul 1;17(13):3619-30
The EMBO journal 1998 Jul 1;17(13):3619-30
Regulation of the pro-B-cell-specific enhancer of the Id1 gene involves the C/EBP family of proteins.
Saisanit S, Sun XH
Molecular and cellular biology 1997 Feb;17(2):844-50
Molecular and cellular biology 1997 Feb;17(2):844-50
Regulation of the pro-B-cell-specific enhancer of the Id1 gene involves the C/EBP family of proteins.
Saisanit S, Sun XH
Molecular and cellular biology 1997 Feb;17(2):844-50
Molecular and cellular biology 1997 Feb;17(2):844-50
TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling.
Zinszner H, Sok J, Immanuel D, Yin Y, Ron D
Journal of cell science 1997 Aug;110 ( Pt 15):1741-50
Journal of cell science 1997 Aug;110 ( Pt 15):1741-50
Stress-induced phosphorylation and activation of the transcription factor CHOP (GADD153) by p38 MAP Kinase.
Wang XZ, Ron D
Science (New York, N.Y.) 1996 May 31;272(5266):1347-9
Science (New York, N.Y.) 1996 May 31;272(5266):1347-9
Stress-induced phosphorylation and activation of the transcription factor CHOP (GADD153) by p38 MAP Kinase.
Wang XZ, Ron D
Science (New York, N.Y.) 1996 May 31;272(5266):1347-9
Science (New York, N.Y.) 1996 May 31;272(5266):1347-9
Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153).
Wang XZ, Lawson B, Brewer JW, Zinszner H, Sanjay A, Mi LJ, Boorstein R, Kreibich G, Hendershot LM, Ron D
Molecular and cellular biology 1996 Aug;16(8):4273-80
Molecular and cellular biology 1996 Aug;16(8):4273-80
Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153).
Wang XZ, Lawson B, Brewer JW, Zinszner H, Sanjay A, Mi LJ, Boorstein R, Kreibich G, Hendershot LM, Ron D
Molecular and cellular biology 1996 Aug;16(8):4273-80
Molecular and cellular biology 1996 Aug;16(8):4273-80
Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153).
Batchvarova N, Wang XZ, Ron D
The EMBO journal 1995 Oct 2;14(19):4654-61
The EMBO journal 1995 Oct 2;14(19):4654-61
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.