20485-1-AP

antibody from Proteintech Group

Targeting: TMEM165 GDT1, TMPT27, TPARL

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

20485-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20485-1-AP, RRID:AB_10695184

Product name

TMEM165 antibody

Antibody type

Polyclonal

Description

TMEM165 antibody (Cat. #20485-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

A mechanism of lysosomal calcium entry.

Zajac M, Mukherjee S, Anees P, Oettinger D, Henn K, Srikumar J, Zou J, Saminathan A, Krishnan Y
Science advances 2024 Feb 16;10(7):eadk2317

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Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma.

Lin S, Cao Y, Zhu K, Yang C, Zhu X, Zhang H, Zhang R
Journal of hepatocellular carcinoma 2023;10:1749-1765

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Manganese regulation of COPII condensation controls circulating lipid homeostasis.

Wang X, Huang R, Wang Y, Zhou W, Hu Y, Yao Y, Cheng K, Li X, Xu B, Zhang J, Xu Y, Zeng F, Zhu Y, Chen XW
Nature cell biology 2023 Nov;25(11):1650-1663

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Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.

Nthiga TM, Shrestha BK, Bruun JA, Larsen KB, Lamark T, Johansen T
The Journal of cell biology 2021 Jun 7;220(6)

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The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells.

Stribny J, Thines L, Deschamps A, Goffin P, Morsomme P
The Journal of biological chemistry 2020 Mar 20;295(12):3865-3874

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TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity.

Lee JS, Kim MY, Park ER, Shen YN, Jeon JY, Cho EH, Park SH, Han CJ, Choi DW, Jang JJ, Suh KS, Hong J, Kim SB, Lee KH
Oncology reports 2018 Sep;40(3):1297-1306

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The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca(2+) ATPase (SPCA1).

Reinhardt TA, Lippolis JD, Sacco RE
Biochemical and biophysical research communications 2014 Mar 7;445(2):417-21

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP(TMEM165 antibody) at dilution of 1:3000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The TMEM165 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human TMEM165. This antibody recognizes human,mouse,rat antigen. The TMEM165 antibody has been validated for the following applications: ELISA, IF, IP, WB analysis.

Appendix

20485-1-AP.pdf

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Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 3440ug.

Sample type

cell line