20485-1-AP

antibody from Proteintech Group

Targeting: TMEM165 GDT1, TMPT27, TPARL

Provider product page for 20485-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [12]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]

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Product number: 20485-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20485-1-AP, RRID:AB_10695184
Product name: TMEM165 antibody
Antibody type: Polyclonal
Description: TMEM165 antibody (Cat. #20485-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

TMEM165 protein structure - 20485-1-AP shown in red.

Submitted references TMEM165 promotes glioblastoma progression through epithelial-mesenchymal transition-mediated invasion and temozolomide chemoresistance.

Cheng H, Ding Y, Shen X, Wang Q, Zhang Z, Wang Y, Chen S, Zhuo S, Wang K, Yang L, Ji F, Fu Z, Lan Q, Yang K
International journal of biological macromolecules 2026 Jan;335(Pt 1):149216

TM9SF3 is a Golgi-resident ATG8-binding protein essential for Golgi-selective autophagy.

Yang J, Dong Y, Xu J, Qian X, Cai Y, Chen Y, Zhang P, Gao D, Cui Z, Cui Y
Developmental cell 2025 Nov 3;60(21):2862-2879.e8

Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca(2+) import and H(+) efflux.

Chen R, Liu B, Jaślan D, Kucej L, Kudrina V, Warnke B, Klingl YE, Petrauskas A, Castro SP, Maeda K, Grimm C, Jäättelä M
Nature communications 2025 Jun 5;16(1):5209

Golgi pH homeostasis stabilizes the lysosomal membrane through N-glycosylation of membrane proteins.

Sou YS, Yamaguchi J, Masuda K, Uchiyama Y, Maeda Y, Koike M
Life science alliance 2024 Oct;7(10)

YIPF3 and YIPF4 regulate autophagic turnover of the Golgi apparatus.

Kitta S, Kaminishi T, Higashi M, Shima T, Nishino K, Nakamura N, Kosako H, Yoshimori T, Kuma A
The EMBO journal 2024 Jul;43(14):2954-2978

A mechanism of lysosomal calcium entry.

Zajac M, Mukherjee S, Anees P, Oettinger D, Henn K, Srikumar J, Zou J, Saminathan A, Krishnan Y
Science advances 2024 Feb 16;10(7):eadk2317

Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma.

Lin S, Cao Y, Zhu K, Yang C, Zhu X, Zhang H, Zhang R
Journal of hepatocellular carcinoma 2023;10:1749-1765

Manganese regulation of COPII condensation controls circulating lipid homeostasis.

Wang X, Huang R, Wang Y, Zhou W, Hu Y, Yao Y, Cheng K, Li X, Xu B, Zhang J, Xu Y, Zeng F, Zhu Y, Chen XW
Nature cell biology 2023 Nov;25(11):1650-1663

Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.

Nthiga TM, Shrestha BK, Bruun JA, Larsen KB, Lamark T, Johansen T
The Journal of cell biology 2021 Jun 7;220(6)

The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells.

Stribny J, Thines L, Deschamps A, Goffin P, Morsomme P
The Journal of biological chemistry 2020 Mar 20;295(12):3865-3874

TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity.

Lee JS, Kim MY, Park ER, Shen YN, Jeon JY, Cho EH, Park SH, Han CJ, Choi DW, Jang JJ, Suh KS, Hong J, Kim SB, Lee KH
Oncology reports 2018 Sep;40(3):1297-1306

The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca(2+) ATPase (SPCA1).

Reinhardt TA, Lippolis JD, Sacco RE
Biochemical and biophysical research communications 2014 Mar 7;445(2):417-21

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP(TMEM165 antibody) at dilution of 1:3000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The TMEM165 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human TMEM165. This antibody recognizes human,mouse,rat antigen. The TMEM165 antibody has been validated for the following applications: ELISA, IF, IP, WB analysis.
Appendix: 20485-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 3440ug.
Sample type: cell line