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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6475 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PAST1 Antibody / EHD1 / EH domain-containing protein 1
- Antibody type
- Polyclonal
- Description
- This highly specific PAST1 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human and mouse, monkey samples.
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PAST1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human T47-D, 2) human Caco-2, 3) human A549, 4) human MCF7, 5) monkey COS-7, 6) mouse testis, 7) mouse kidney, 8) mouse lung and 9) mouse ovary lysate with PAST1 antibody. Expected molecular weight ~61 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human SiHa cells with PAST1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human SiHa cells with PAST1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAST1 antibody.
