ABIN362345

antibody from antibodies-online

Targeting: CTTN EMS1

Western blot (Recommended by provider)

Antibody Data

Product number

ABIN362345

Provider

antibodies-online

Product name

anti-Cortactin (CTTN) (pTyr466) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pTyr466 (P-V-Y (p) -E-T) derived from Human CORTACTIN. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse

Host

Rabbit

Epitope

pTyr466

Isotype

IgG

Vial size

50 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

CXCL12 induces tyrosine phosphorylation of cortactin, which plays a role in CXC chemokine receptor 4-mediated extracellular signal-regulated kinase activation and chemotaxis.

Luo C, Pan H, Mines M, Watson K, Zhang J, Fan GH
The Journal of biological chemistry 2006 Oct 6;281(40):30081-93

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Cortactin interacts with WIP in regulating Arp2/3 activation and membrane protrusion.

Kinley AW, Weed SA, Weaver AM, Karginov AV, Bissonette E, Cooper JA, Parsons JT
Current biology : CB 2003 Mar 4;13(5):384-93

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Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton.

Head JA, Jiang D, Li M, Zorn LJ, Schaefer EM, Parsons JT, Weed SA
Molecular biology of the cell 2003 Aug;14(8):3216-29

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Tyrosine phosphorylation of cortactin is required for H2O2-mediated injury of human endothelial cells.

Li Y, Liu J, Zhan X
The Journal of biological chemistry 2000 Nov 24;275(47):37187-93