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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-29799 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cortactin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Neuro 2A, C8D30, NIH-3T3, C2C12, PC-12, Rat2, mouse brain, rat brain.
- Concentration
- 0.28 mg/mL
Submitted references Metabolic control of the scaffold protein TKS5 in tissue-invasive, proinflammatory T cells.
Shen Y, Wen Z, Li Y, Matteson EL, Hong J, Goronzy JJ, Weyand CM
Nature immunology 2017 Sep;18(9):1025-1034
Nature immunology 2017 Sep;18(9):1025-1034
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cortactin using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Cortactin polyclonal antibody (Product # PA5-29799) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cortactin was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cortactin Polyclonal Antibody (Product # PA5-29799) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cortactin was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cortactin Polyclonal Antibody (Product # PA5-29799) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Cortactin was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cortactin Polyclonal Antibody (Product # PA5-29799) at a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cortactin in methanol-fixed HeLa cells using a Cortactin polyclonal antibody (Product # PA5-29799) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Cortactin was performed in paraffin-embedded mouse intestine tissue using Cortactin Polyclonal Antibody (Product # PA5-29799) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cortactin (Product # PA5-29799) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
