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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB5262 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Fas Ligand/TNFSF6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Fas Ligand/TNFSF6 in ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant mouse (rm) TNF-alpha is observed and no cross-reactivity with recombinant human (rh) APRIL, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTRAIL, rmTRANCE, and rhVEGI is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P41047- Isotype
- IgG
- Antibody clone number
- 101626
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references FasL Modulates Expression of Mmp2 in Osteoblasts.
Glycolysis promotes caspase-3 activation in lipid rafts in T cells.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Antigen-specific CD25- Foxp3- IFN-gamma(high) CD4+ T cells restrain the development of experimental allergic encephalomyelitis by suppressing Th17.
Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival.
Svandova E, Vesela B, Lesot H, Sadoine J, Poliard A, Matalova E
Frontiers in physiology 2018;9:1314
Frontiers in physiology 2018;9:1314
Glycolysis promotes caspase-3 activation in lipid rafts in T cells.
Secinaro MA, Fortner KA, Dienz O, Logan A, Murphy MP, Anathy V, Boyson JE, Budd RC
Cell death & disease 2018 Jan 19;9(2):62
Cell death & disease 2018 Jan 19;9(2):62
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Antigen-specific CD25- Foxp3- IFN-gamma(high) CD4+ T cells restrain the development of experimental allergic encephalomyelitis by suppressing Th17.
Wildbaum G, Zohar Y, Karin N
The American journal of pathology 2010 Jun;176(6):2764-75
The American journal of pathology 2010 Jun;176(6):2764-75
Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival.
Krum SA, Miranda-Carboni GA, Hauschka PV, Carroll JS, Lane TF, Freedman LP, Brown M
The EMBO journal 2008 Feb 6;27(3):535-45
The EMBO journal 2008 Feb 6;27(3):535-45
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by Fas Ligand/TNFSF6 and Neutralization by Mouse Fas Ligand/TNFSF6 Antibody. In the presence of a cross-linking antibody, Mouse polyHisti-dine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recom-binant Mouse Fas Ligand/ TNFSF6 (Catalog # 526-SA) induces cytotoxicity in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Mouse Fas Ligand/ TNFSF6 (5 µg/mL) is neutral-ized (green line) by increasing concentrations of Rat Anti-Mouse Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB5262). The ND50 is typically 5-20 µg/mL.
