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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB1858 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat Fas Ligand/TNFSF6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat Fas Ligand/TNFSF6 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB Ligand, recombinant human (rh) APRIL, rmBAFF, rhEDA-A2, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, rmLIGHT, rhLymphotoxin alpha 1/beta 2, rhLymphotoxin alpha 2/beta 1, rmOX40Ligand, rhTL1, recombinant cotton rat TNF-alpha , rmTNF-alpha , rrTNF-alpha , rmTrail, rhTrance, and rmTWEAK is observed.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P36940- Isotype
- IgG
- Antibody clone number
- 252029
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Fas Ligand/TNFSF6 in Rat Splenocytes. Fas Ligand/TNFSF6 was detected in immersion fixed rat splenocytes using Mouse Anti-Rat Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB1858) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
