Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF526 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Fas Ligand/TNFSF6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Fas Ligand/TNFSF6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fas Ligand is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q544E9
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
Murata T, Tsuboi M, Hikita K, Kaneda N
The Journal of biological chemistry 2006 Aug 11;281(32):22503-16
The Journal of biological chemistry 2006 Aug 11;281(32):22503-16
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by Fas Ligand/TNFSF6 and Neutralization by Mouse Fas Ligand/TNFSF6 Antibody. In the presence of a cross-linking antibody, mouse anti-HA Monoclonal Antibody, Recombinant Mouse Fas Ligand/TNFSF6 (Catalog # 6128-SA) induces cytotoxicity in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Mouse Fas Ligand/TNFSF6 (10 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse Fas Ligand/ TNFSF6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF526). The ND50 is typically 0.125-0.75 µg/mL.