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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- DM1212 - Provider product page
- Provider
- OriGene
- Product name
- Fas Ligand (FASLG) mouse monoclonal antibody, clone GM5F4, Purified
- Antibody type
- Monoclonal
- Description
- Fas Ligand (FASLG) mouse monoclonal antibody, clone GM5F4, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FASLG
- Isotype
- IgG
- Antibody clone number
- GM5F4
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Fig.2: SDS-PAGE analysis of purified GM-5F4 monoclonalantibody. Lane 1: molecular weight marker, Lane 2: 2 ug ofpurified GM-5F4 antibody. Proteins were separated by SDS-PAGE and stained with RAPID Stain(TM) Reagent.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Fig.1: FACS analysis of BOSC23 cells using GM-5F4 . BOSC23 cells were transiently transfected with an expres-sion vector encoding either FasL (red curve) or an irrelevantprotein (control transfectant: black curve). Binding of GM-5F4wasdetected with a PE-conjugated secondary antibody. A positivesignal was obtained only with FasL transfected cells.
- Validation comment
- FC
