Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 12-9919-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended. Applications Reported: NOK-1 has been reported for use in flow cytometric analysis. Applications Tested: This NOK-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- NOK-1
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion.
CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity.
Silencing of soluble epoxide hydrolase 2 gene reduces H(2)O(2)-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.
Interleukin-24 Regulates T Cell Activity in Patients With Colorectal Adenocarcinoma.
Transmembrane TNF-α promotes activation-induced cell death by forward and reverse signaling.
IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.
Long-term effect on natural killer cells by interferon-α therapy on the outcomes of HCV infection.
Fas-mediated killing of primary prostate cancer cells is increased by mitoxantrone and docetaxel.
Siva-1 negatively regulates NF-kappaB activity: effect on T-cell receptor-mediated activation-induced cell death (AICD).
c-FLIP inhibits chemotherapy-induced colorectal cancer cell death.
Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing.
Expression of the functional soluble form of human fas ligand in activated lymphocytes.
Metalloproteinase-mediated release of human Fas ligand.
Myers JA, Schirm D, Bendzick L, Hopps R, Selleck C, Hinderlie P, Felices M, Miller JS
JCI insight 2022 Aug 8;7(15)
JCI insight 2022 Aug 8;7(15)
CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity.
Bernareggi D, Xie Q, Prager BC, Yun J, Cruz LS, Pham TV, Kim W, Lee X, Coffey M, Zalfa C, Azmoon P, Zhu H, Tamayo P, Rich JN, Kaufman DS
Nature communications 2022 Apr 7;13(1):1899
Nature communications 2022 Apr 7;13(1):1899
Silencing of soluble epoxide hydrolase 2 gene reduces H(2)O(2)-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.
Li J, Luo J, Zhang Y, Tang C, Wang J, Chen C
Cytotechnology 2020 Feb;72(1):23-36
Cytotechnology 2020 Feb;72(1):23-36
Interleukin-24 Regulates T Cell Activity in Patients With Colorectal Adenocarcinoma.
Zhang Y, Liu Y, Xu Y
Frontiers in oncology 2019;9:1401
Frontiers in oncology 2019;9:1401
Transmembrane TNF-α promotes activation-induced cell death by forward and reverse signaling.
Zhang M, Wang J, Jia L, Huang J, He C, Hu F, Yuan L, Wang G, Yu M, Li Z
Oncotarget 2017 Sep 8;8(38):63799-63812
Oncotarget 2017 Sep 8;8(38):63799-63812
IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.
Braun M, Ress ML, Yoo YE, Scholz CJ, Eyrich M, Schlegel PG, Wölfl M
Oncoimmunology 2016 Jul;5(7):e1188245
Oncoimmunology 2016 Jul;5(7):e1188245
Long-term effect on natural killer cells by interferon-α therapy on the outcomes of HCV infection.
Hu X, Jiang Y, Li X, Gao Y, Guo X, Chi X, Yan H, Feng J, Zhong J, Sun B, Shao X, Xiao W, Pan Y, Niu J
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 May;34(5):366-75
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2014 May;34(5):366-75
Fas-mediated killing of primary prostate cancer cells is increased by mitoxantrone and docetaxel.
Symes JC, Kurin M, Fleshner NE, Medin JA
Molecular cancer therapeutics 2008 Sep;7(9):3018-28
Molecular cancer therapeutics 2008 Sep;7(9):3018-28
Siva-1 negatively regulates NF-kappaB activity: effect on T-cell receptor-mediated activation-induced cell death (AICD).
Gudi R, Barkinge J, Hawkins S, Chu F, Manicassamy S, Sun Z, Duke-Cohan JS, Prasad KV
Oncogene 2006 Jun 8;25(24):3458-62
Oncogene 2006 Jun 8;25(24):3458-62
c-FLIP inhibits chemotherapy-induced colorectal cancer cell death.
Longley DB, Wilson TR, McEwan M, Allen WL, McDermott U, Galligan L, Johnston PG
Oncogene 2006 Feb 9;25(6):838-48
Oncogene 2006 Feb 9;25(6):838-48
Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing.
Suda T, Hashimoto H, Tanaka M, Ochi T, Nagata S
The Journal of experimental medicine 1997 Dec 15;186(12):2045-50
The Journal of experimental medicine 1997 Dec 15;186(12):2045-50
Expression of the functional soluble form of human fas ligand in activated lymphocytes.
Tanaka M, Suda T, Takahashi T, Nagata S
The EMBO journal 1995 Mar 15;14(6):1129-35
The EMBO journal 1995 Mar 15;14(6):1129-35
Metalloproteinase-mediated release of human Fas ligand.
Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, Yagita H
The Journal of experimental medicine 1995 Dec 1;182(6):1777-83
The Journal of experimental medicine 1995 Dec 1;182(6):1777-83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of human FasL tranfected cells with Anti-Human CD178 (CD95 Ligand) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EVs secreted by Cal27 induce apoptosis in NK cells. a Cal27-WT and KO derived EVs were analyzed by flow cytometry for known NKG2D ligands MICA/B, ULBP2/5/6, ULPB3, and for TRAIL and FasL, proteins involved in intrinsic or extrinsic apoptosis pathway (green histograms). In blue is shown the isotype control. Representative of n = 2 independent experiments. b Flow cytometry CASP3/7 viability assay showing increasing NK cell death once exposed to EVs from Cal27 in a time course of 4-hours. The error bars represent +-SEM across n = 3 replicates. Two-way ANOVA and Bonferroni's post hoc test was performed to determine statistical significance. Representative of n = 3 independent experiments. c 24 hour time course viability assay quantified using the IncuCyte real-time imaging system. N = 5 images/well taken for each of three replicates. The error bars represent +-SEM across n = 3 replicates. Statistical analysis was performed by two-way ANOVA and Bonferroni's post hoc multiple comparison test. Representative of n = 2 independent experiments. d 4 hour cytotoxicity assay using NK cells as effectors against Cal27-WT or KO as target cells. Here we show how Cal27-derived EVs reduce the killing in Cal27-KO. Error bars represent +-SEM across n = 3 replicates. Statistical analysis was performed by one-way ANOVA and Bonferroni's post hoc test (ns, not significant). e antibodies blocking MICA/B, TRAIL, or a combination of the two (aMICA/B and aTRAIL) limit the inhibitory effect of EV on NK ce
- Conjugate
- Yellow dye