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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 306409 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#306409, RRID:AB_2293687
- Product name
- LEAF™ Purified anti-human CD178 (Fas-L) [NOK-1]
- Antibody type
- Monoclonal
- Antigen
- L5178Y mouse T lymphoma cells expressing recombinant human FasL
- Description
- CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 1 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
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- Experimental details
- Human Fas Ligand transfected cells stained with LEAF™ purified NOK-1, followed by biotinylated anti-mouse IgG and Sav-PE
- Conjugate
- Unconjugated
