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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- APRab10837 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- FAS-L Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purification
- Reactivity
- Human, Mouse, Other
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Polyclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from 1)HepG2, 2)293 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β monoclonal antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody was diluted at 1:10000, 37° 1hour.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western Blot analysis of various cells using FAS-L Polyclonal Antibody diluted at 1:1000
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western Blot analysis of 293 cells using FAS-L Polyclonal Antibody diluted at 1:1000
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of rat-kidney tissue. 1,FAS-L Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of mouse-kidney tissue. 1,FAS-L Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,FAS-L Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,FAS-L Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
