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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB126 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fas Ligand/TNFSF6 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human Fas Ligand/TNFSF6 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse Fas Ligand is observed and less than 1% cross-reactivity with recombinant human (rh) GITR Ligand, rhTNF-alpha , and rhTRAIL is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P48023- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Apoptosis Induced by Fas Ligand/TNFSF6 and Neutralization by Human Fas Ligand/TNFSF6 Antibody. Recombinant Human Fas Ligand/ TNFSF6 (Catalog # 126-FL) induces apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Apoptosis elicited by Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Fas Ligand/ TNFSF6 Polyclonal Antibody (Catalog # AB126). The ND50 is typically 0.05-0.2 µg/mL.
