15-0339-42

antibody from Invitrogen Antibodies
Targeting: CD33 FLJ00391, p67, SIGLEC-3, SIGLEC3
Provider product page for 15-0339-42

Antibody data

Product number
15-0339-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD33 Monoclonal Antibody (HIM3-4), PE-Cyanine5, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion. Applications Reported: The HIM3-4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIM3-4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
HIM3-4
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4°C, store in dark, DO NOT FREEZE!
CD33 protein structure - 15-0339-42 shown in red.