Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-4064 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-CD33Antibody
- Antibody type
- Monoclonal
- Description
- CD33 is a member of the SIGLEC (Sialic Acid-Binding Ig-Like Lectin) family of receptors, and the gene comprises seven coding exons. Exon 2 encodes the canonical IgV domain, exon 4 encodes the IgC structural domain, and exons 6 and 7 encode cytosolic ITIMs (Immunotyrosine Inhibitory Motifs). CD33 acts as a cell surface antigen which is expressed on normal myeloid cells and CD34+ blasts in AML (Acute Myeloid Leukemia). The antigen serves as a target of GO (Gemtuzumab/Ozogamicin), which exerts anti-leukemic effects in refractory AML.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant CD33 p
rotein (amino acids 28-320) was use
d as the immunogen for this antibod
y.- Isotype
- IgG
- Antibody clone number
- ABM29D3
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CD33. Anti- CD33 antibody (Clone: ABM29D3) was used at 2 µg/ml on Human Spleen and DU145 lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD33 in adenocarcinoma of rectum using CD33 antibody (Clone: ABM29D3) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD33 in Renal Cell Carcinoma using CD33 antibody (Clone: ABM29D3) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD33 in squamous cell carcinoma of esophagus using CD33 antibody (Clone: ABM29D3) at 5 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Cell Surface flow analysis of hCD33 in PBMC (Monocytes) using 0.2µg/10^6 cells of CD33 clone ( ABM29D3). Green represents isotype control; red represents anti-hCD33 antibody. Goat anti-mouse PE conjugated secondary antibody (ABEOMICS) was used.
- Protocol
- Protocol