H00007852-M05

antibody from Abnova Corporation

Targeting: CXCR4 CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007852-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007852-M05, RRID:AB_606116

Product name

CXCR4 monoclonal antibody (M05), clone 2F1

Antibody type

Monoclonal

Antigen

CXCR4 (AAH20968, 1 a.a. ~ 46 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Reactivity

Human

Host

Mouse

Antigen sequence

MEGISIYTSDNYTEEMGSGDYDSMKEPCFREENAN
FNKIFLPTIYS


Isotype

IgG

Vial size

50 µg

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

CXCR4 is highly expressed at the tumor front but not in the center of prostate cancers.

Delongchamps NB, Beuvon F, Mathieu JR, Delmas S, Metzger I, Prats H, Cabon F
World journal of urology 2015 Feb;33(2):281-7

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CXCR7 expression is associated with disease-free and disease-specific survival in cervical cancer patients.

Schrevel M, Karim R, ter Haar NT, van der Burg SH, Trimbos JB, Fleuren GJ, Gorter A, Jordanova ES
British journal of cancer 2012 Apr 24;106(9):1520-5

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CXCR4 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to CXCR4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol