PA5-19857

antibody from Invitrogen Antibodies

Targeting: CXCR4 CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19857

Provider

Invitrogen Antibodies

Product name

CXCR4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is Hela cell lysate. PA5-19857 can be used with blocking peptide PEP-0002. The PA5-19857 immunogen is located within amino acids 170-220 of CXCR4. Predicted molecular ~ 40kD. In Western blot applications, this antibody has been observed to detect a band at: 44kD (Post-modification: 2 N-linked glycosylation) Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (86%), Sheep: (86%), Mouse: (73%), Rat: (67%)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

Cigarette smoke promotes HIV infection of primary bronchial epithelium and additively suppresses CFTR function.

Chinnapaiyan S, Dutta R, Bala J, Parira T, Agudelo M, Nair M, Unwalla HJ
Scientific reports 2018 May 22;8(1):7984

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HeLa total cell lysate using a CD184/CXCR4 polyclonal antibody (Product # PA5-19857) at 1:1000 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 CS increases expression of HIV receptors CD4 and CCR5 in NHBE ALI cultures. NHBE ALI cultures were exposed to CS and total protein was extracted. Cells were lysed with RIPA buffer containing protease inhibitor cocktail and protein expression was quantified by Western blot analysis and normalized using beta-actin. CS significantly enhances expression of CD4 (panel a) and CCR5 protein expression (panel b) when compared to air-exposed controls. CS does not increase CXCR4 protein expression (panel c). Western blot images ( a - c ) are representative of NHBE ALI cultures from three independent lungs. Relative density of the detected protein band was measured by using the ImageJ software and the values obtained were averaged. n = 3 lungs (unless stated otherwise). *Significant (p < 0.05).