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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-78601 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GFM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, HepG2 Predicted Reactivity: Rat (84%), Rhesus Monkey (97%), Bovine (92%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GFM2 in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with GFM2 polyclonal antibody (Product # PA5-78601) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GFM2 Polyclonal Antibody detects GFM2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GFM2 Polyclonal Antibody (Product # PA5-78601) diluted at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GFM2 Polyclonal Antibody detects GFM2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: GFM2 protein stained by GFM2 Polyclonal Antibody (Product # PA5-78601) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GFM2 Polyclonal Antibody detects GFM2 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GFM2 Polyclonal Antibody (Product # PA5-78601) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
