NBP2-22115

antibody from Novus Biologicals

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Provider product page for NBP2-22115

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [13]
Comments [0]
Validations
Western blot [2]
ELISA [1]
Immunohistochemistry [2]
Flow cytometry [1]

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Product number: NBP2-22115 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP2-22115, RRID:AB_2728754
Product name: Mouse Monoclonal CCL2/MCP1 Antibody
Antibody type: Monoclonal
Description: Ammonium sulfate precipitation.
Reactivity: Human, Mouse, Rat, Feline, Simian
Host: Mouse
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1.0 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

CCL2 protein structure - NBP2-22115 shown in red.

Submitted references DHEAS prevents pro-metastatic and proliferative effects of 17ß-estradiol on MCF-7 breast cancer cells.

Upmanyu N, Bulldan A, Failing K, Scheiner-Bobis G
Biochimica et biophysica acta. Molecular cell research 2020 Feb;1867(2):118600

Nephrogenic Systemic Fibrosis Is Mediated by Myeloid C-C Chemokine Receptor 2.

Do C, Drel V, Tan C, Lee D, Wagner B
The Journal of investigative dermatology 2019 Oct;139(10):2134-2143.e2

Salt increases monocyte CCR2 expression and inflammatory responses in humans.

Wenstedt EF, Verberk SG, Kroon J, Neele AE, Baardman J, Claessen N, Pasaoglu ÖT, Rademaker E, Schrooten EM, Wouda RD, de Winther MP, Aten J, Vogt L, Van den Bossche J
JCI insight 2019 Nov 1;4(21)

LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8⁺ T cell tumor-infiltration impairing anti-PD1 therapy.

Pascual-García M, Bonfill-Teixidor E, Planas-Rigol E, Rubio-Perez C, Iurlaro R, Arias A, Cuartas I, Sala-Hojman A, Escudero L, Martínez-Ricarte F, Huber-Ruano I, Nuciforo P, Pedrosa L, Marques C, Braña I, Garralda E, Vieito M, Squatrito M, Pineda E, Graus F, Espejo C, Sahuquillo J, Tabernero J, Seoane J
Nature communications 2019 Jun 11;10(1):2416

Direct role of the C-C motif chemokine receptor 2/monocyte chemoattractant protein 1 system in the feline cumulus oocyte complex†.

Rojo JL, Jaworski JP, Peluffo MC
Biology of reproduction 2019 Apr 1;100(4):1046-1056

Immunotopographical Differences of Human Skin.

Béke G, Dajnoki Z, Kapitány A, Gáspár K, Medgyesi B, Póliska S, Hendrik Z, Péter Z, Törőcsik D, Bíró T, Szegedi A
Frontiers in immunology 2018;9:424

Hydroxytyrosol inhibits the inflammatory response of osteoarthritis chondrocytes via SIRT6-mediated autophagy.

Zhi LQ, Yao SX, Liu HL, Li M, Duan N, Ma JB
Molecular medicine reports 2018 Mar;17(3):4035-4042

Macrophages orchestrate breast cancer early dissemination and metastasis.

Linde N, Casanova-Acebes M, Sosa MS, Mortha A, Rahman A, Farias E, Harper K, Tardio E, Reyes Torres I, Jones J, Condeelis J, Merad M, Aguirre-Ghiso JA
Nature communications 2018 Jan 2;9(1):21

A Hematogenous Route for Medulloblastoma Leptomeningeal Metastases.

Garzia L, Kijima N, Morrissy AS, De Antonellis P, Guerreiro-Stucklin A, Holgado BL, Wu X, Wang X, Parsons M, Zayne K, Manno A, Kuzan-Fischer C, Nor C, Donovan LK, Liu J, Qin L, Garancher A, Liu KW, Mansouri S, Luu B, Thompson YY, Ramaswamy V, Peacock J, Farooq H, Skowron P, Shih DJH, Li A, Ensan S, Robbins CS, Cybulsky M, Mitra S, Ma Y, Moore R, Mungall A, Cho YJ, Weiss WA, Chan JA, Hawkins CE, Massimino M, Jabado N, Zapotocky M, Sumerauer D, Bouffet E, Dirks P, Tabori U, Sorensen PHB, Brastianos PK, Aldape K, Jones SJM, Marra MA, Woodgett JR, Wechsler-Reya RJ, Fults DW, Taylor MD
Cell 2018 Feb 22;172(5):1050-1062.e14

CD44 Promotes Inflammation and Extracellular Matrix Production During Arteriovenous Fistula Maturation.

Kuwahara G, Hashimoto T, Tsuneki M, Yamamoto K, Assi R, Foster TR, Hanisch JJ, Bai H, Hu H, Protack CD, Hall MR, Schardt JS, Jay SM, Madri JA, Kodama S, Dardik A
Arteriosclerosis, thrombosis, and vascular biology 2017 Jun;37(6):1147-1156

Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice.

Nishio M, Sugimachi K, Goto H, Wang J, Morikawa T, Miyachi Y, Takano Y, Hikasa H, Itoh T, Suzuki SO, Kurihara H, Aishima S, Leask A, Sasaki T, Nakano T, Nishina H, Nishikawa Y, Sekido Y, Nakao K, Shin-Ya K, Mimori K, Suzuki A
Proceedings of the National Academy of Sciences of the United States of America 2016 Jan 5;113(1):E71-80

Atherosclerosis following renal injury is ameliorated by pioglitazone and losartan via macrophage phenotype.

Yamamoto S, Zhong J, Yancey PG, Zuo Y, Linton MF, Fazio S, Yang H, Narita I, Kon V
Atherosclerosis 2015 Sep;242(1):56-64

T-box transcription factor Brachyury in lung cancer cells inhibits macrophage infiltration by suppressing CCL2 and CCL4 chemokines.

Chen S, Jiao J, Jiang D, Wan Z, Li L, Li K, Xu L, Zhou Z, Xu W, Xiao J
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Aug;36(8):5881-90

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) cell lysates.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa)

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: ELISA: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - HER2 activates NF-kB and upregulates CCL2. MGs from FVB wild-type and 22-week-old MMTV-HER2 mice stained against CCL2, HER2, and CCR2. Bars: 25 um. Inset, CCR2 signal, zoom factor 1x; full image in Supplementary Fig. 5i. CCL2 intensity was quantified using ROI tool in Metamorph (Supplementary Fig. 5h). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-017-02481-5), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CCL2/MCP1 Antibody (2D8) [NBP2-22115] - Analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red).