AF1774

antibody from Novus Biologicals

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1774

Provider

Novus Biologicals

Product name

Goat Polyclonal CCL2/JE/MCP-1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects canine CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) CCL2/MCP-1 is observed, approximately 10% cross-reactivity with recombinant mouse (rm) JE, recombinant rat JE, and recombinant cotton rat JE is observed, and less than 5% cross-reactivity with rhEotaxin, rmMARC, and rhMCP-2 is observed.

Reactivity

Canine

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chemotaxis Induced by CCL2/JE/MCP-1 and Neutralization by Canine CCL2/JE/MCP-1 Antibody. Recombinant Canine CCL2/JE/ MCP-1 (Catalog # 1774-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/ MCP-1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine CCL2/JE/ MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1774). The ND₅₀ is typically 0.8-4.0 µg/mL.