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LearnIC479A
antibody from Novus Biologicals
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC479A - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal CCL2/JE/MCP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CCL2/JE/MCP-1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with LPS for 48 hours were stained with Rat Anti-Mouse CCL2/JE/MCP-1 APC-conjugated Monoclonal Antibody (Catalog # IC479A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
