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LearnTA336914
antibody from OriGene
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA336914 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal CCL2/MCP1 Antibody (2D8)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal CCL2/MCP1 Antibody (2D8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CCL2
- Isotype
- IgG
- Antibody clone number
- 2D8
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: MCP1 Antibody (2D8) [TA336914] - Western blot analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: MCP1 Antibody (2D8) [TA336914] - Western blot analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6)cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ELISA: MCP1 Antibody (2D8) [TA336914] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MCP1 Antibody (2D8) [TA336914] - Immunofluorescence analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCP1 Antibody (2D8) [TA336914] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: MCP1 Antibody (2D8) [TA336914] - Flow cytometric analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red).
- Validation comment
- FC
