GTX48661
antibody from GeneTex
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- GTX48661 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48661, RRID:AB_11172610
- Product name
- MCP1 / CCL2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-human MCP-1 by western blot shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 µg, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's, Inc. Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of Anti-human MCP-1 antibody shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 ?g, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's Anti-Human MCP-1 antibody. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc? 4000 MP imaging system.