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LearnGTX81767
antibody from GeneTex
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX81767 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81767, RRID:AB_11166975
- Product name
- MCP1 / CCL2 antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Astrocytes promote progression of breast cancer metastases to the brain via a KISS1-mediated autophagy.
Kaverina N, Borovjagin AV, Kadagidze Z, Baryshnikov A, Baryshnikova M, Malin D, Ghosh D, Shah N, Welch DR, Gabikian P, Karseladze A, Cobbs C, Ulasov IV
Autophagy 2017;13(11):1905-1923
Autophagy 2017;13(11):1905-1923
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of CCL2 Antibody (C-term) (GTX81767) in Hela cell line lysates (35μg/lane). CCL2 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CCL2 Antibody (C-term)(GTX81767) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjμgated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of CCL2 Antibody (C-term) (GTX81767) with Hela cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue with CCL2 Antibody (C-term)(GTX81767), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of hela cells using CCL2 Antibody (C-term)(bottom histogram)(GTX81767) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
