MAB679-100
antibody from R&D Systems
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB679-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL2/JE/MCP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL2/JE/MCP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) CCL2, 3, 4, rhCCL3, 4, 5, 7, or 8.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 23007
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1β and MCP-1 in cultured human retinal pigment epithelial cells.
CCL2 influences the sensitivity of lung cancer A549 cells to docetaxel.
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis.
Evidence for chemokine synergy during neutrophil migration in ARDS.
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
CCL2 as a potential therapeutic target for clear cell renal cell carcinoma.
Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus.
Microparticles engineered to highly express peroxisome proliferator-activated receptor-γ decreased inflammatory mediator production and increased adhesion of recipient monocytes.
A multiplex immunoassay for human adipokine profiling.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Ultrasensitive flow-based immunoassays using single-molecule counting.
Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
trans fatty acids and systemic inflammation in heart failure.
Bian ZM, Field MG, Elner SG, Kahlenberg JM, Elner VM
Experimental eye research 2018 May;170:29-39
Experimental eye research 2018 May;170:29-39
CCL2 influences the sensitivity of lung cancer A549 cells to docetaxel.
Wang T, Zhan Q, Peng X, Qiu Z, Zhao T
Oncology letters 2018 Jul;16(1):1267-1274
Oncology letters 2018 Jul;16(1):1267-1274
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Gril B, Paranjape AN, Woditschka S, Hua E, Dolan EL, Hanson J, Wu X, Kloc W, Izycka-Swieszewska E, Duchnowska R, Pęksa R, Biernat W, Jassem J, Nayyar N, Brastianos PK, Hall OM, Peer CJ, Figg WD, Pauly GT, Robinson C, Difilippantonio S, Bialecki E, Metellus P, Schneider JP, Steeg PS
Nature communications 2018 Jul 13;9(1):2705
Nature communications 2018 Jul 13;9(1):2705
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑α.
Liu B, Xu L, Yu X, Li W, Sun X, Xiao S, Guo M, Wang H
Molecular medicine reports 2018 Aug;18(2):1987-1994
Molecular medicine reports 2018 Aug;18(2):1987-1994
Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis.
van Sleen Y, Wang Q, van der Geest KSM, Westra J, Abdulahad WH, Heeringa P, Boots AMH, Brouwer E
Scientific reports 2017 Jul 26;7(1):6553
Scientific reports 2017 Jul 26;7(1):6553
Evidence for chemokine synergy during neutrophil migration in ARDS.
Williams AE, José RJ, Mercer PF, Brealey D, Parekh D, Thickett DR, O'Kane C, McAuley DF, Chambers RC
Thorax 2017 Jan;72(1):66-73
Thorax 2017 Jan;72(1):66-73
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Henry CM, Martin SJ
Molecular cell 2017 Feb 16;65(4):715-729.e5
Molecular cell 2017 Feb 16;65(4):715-729.e5
CCL2 as a potential therapeutic target for clear cell renal cell carcinoma.
Arakaki R, Yamasaki T, Kanno T, Shibasaki N, Sakamoto H, Utsunomiya N, Sumiyoshi T, Shibuya S, Tsuruyama T, Nakamura E, Ogawa O, Kamba T
Cancer medicine 2016 Oct;5(10):2920-2933
Cancer medicine 2016 Oct;5(10):2920-2933
Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus.
Connelly KL, Kandane-Rathnayake R, Hoi A, Nikpour M, Morand EF
Scientific reports 2016 Jul 25;6:29909
Scientific reports 2016 Jul 25;6:29909
Microparticles engineered to highly express peroxisome proliferator-activated receptor-γ decreased inflammatory mediator production and increased adhesion of recipient monocytes.
Sahler J, Woeller CF, Phipps RP
PloS one 2014;9(11):e113189
PloS one 2014;9(11):e113189
A multiplex immunoassay for human adipokine profiling.
Schipper HS, de Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clinical chemistry 2010 Aug;56(8):1320-8
Clinical chemistry 2010 Aug;56(8):1320-8
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Ultrasensitive flow-based immunoassays using single-molecule counting.
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clinical chemistry 2007 Nov;53(11):1990-5
Clinical chemistry 2007 Nov;53(11):1990-5
Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Zhao Z, McCloud B, Fleming R, Klempner MS
Biochemical and biophysical research communications 2007 Jun 29;358(2):528-33
Biochemical and biophysical research communications 2007 Jun 29;358(2):528-33
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damås JK
Blood 2006 Aug 1;108(3):928-35
Blood 2006 Aug 1;108(3):928-35
Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
Cardiovascular research 2004 Sep 1;63(4):617-24
Cardiovascular research 2004 Sep 1;63(4):617-24
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Øynebråten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Blood 2004 Jul 15;104(2):314-20
Blood 2004 Jul 15;104(2):314-20
trans fatty acids and systemic inflammation in heart failure.
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL2/JE/MCP-1 in Human Crohn's Disease Intestine. CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL2/MCP-1 and Neutralization by Human CCL2/MCP-1 Antibody. Recombinant Human CCL2/MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679). The ND50 is typically 0.5-2.0 µg/mL.