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LearnMAB479-100
antibody from R&D Systems
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB479-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL2/JE/MCP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human (rh) CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rmCCL1, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P10148- Isotype
- IgG
- Antibody clone number
- 123616
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids.
Myeloid Cells That Impair Immunotherapy Are Restored in Melanomas with Acquired Resistance to BRAF Inhibitors.
Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor.
Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
Near-infrared laser adjuvant for influenza vaccine.
Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.
Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1.
Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization.
MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice.
The role for monocyte chemoattractant protein-1 in the generation and function of memory CD8+ T cells.
Upregulation of VEGF in murine retina via monocyte recruitment after retinal scatter laser photocoagulation.
CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
Role of monocyte chemotactic protein-1/CC chemokine ligand 2 on gamma delta T lymphocyte trafficking during inflammation induced by lipopolysaccharide or Mycobacterium bovis bacille Calmette-Guérin.
Jenkins RW, Aref AR, Lizotte PH, Ivanova E, Stinson S, Zhou CW, Bowden M, Deng J, Liu H, Miao D, He MX, Walker W, Zhang G, Tian T, Cheng C, Wei Z, Palakurthi S, Bittinger M, Vitzthum H, Kim JW, Merlino A, Quinn M, Venkataramani C, Kaplan JA, Portell A, Gokhale PC, Phillips B, Smart A, Rotem A, Jones RE, Keogh L, Anguiano M, Stapleton L, Jia Z, Barzily-Rokni M, Cañadas I, Thai TC, Hammond MR, Vlahos R, Wang ES, Zhang H, Li S, Hanna GJ, Huang W, Hoang MP, Piris A, Eliane JP, Stemmer-Rachamimov AO, Cameron L, Su MJ, Shah P, Izar B, Thakuria M, LeBoeuf NR, Rabinowits G, Gunda V, Parangi S, Cleary JM, Miller BC, Kitajima S, Thummalapalli R, Miao B, Barbie TU, Sivathanu V, Wong J, Richards WG, Bueno R, Yoon CH, Miret J, Herlyn M, Garraway LA, Van Allen EM, Freeman GJ, Kirschmeier PT, Lorch JH, Ott PA, Hodi FS, Flaherty KT, Kamm RD, Boland GM, Wong KK, Dornan D, Paweletz CP, Barbie DA
Cancer discovery 2018 Feb;8(2):196-215
Cancer discovery 2018 Feb;8(2):196-215
Myeloid Cells That Impair Immunotherapy Are Restored in Melanomas with Acquired Resistance to BRAF Inhibitors.
Steinberg SM, Shabaneh TB, Zhang P, Martyanov V, Li Z, Malik BT, Wood TA, Boni A, Molodtsov A, Angeles CV, Curiel TJ, Whitfield ML, Turk MJ
Cancer research 2017 Apr 1;77(7):1599-1610
Cancer research 2017 Apr 1;77(7):1599-1610
Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor.
Rothhammer V, Mascanfroni ID, Bunse L, Takenaka MC, Kenison JE, Mayo L, Chao CC, Patel B, Yan R, Blain M, Alvarez JI, Kébir H, Anandasabapathy N, Izquierdo G, Jung S, Obholzer N, Pochet N, Clish CB, Prinz M, Prat A, Antel J, Quintana FJ
Nature medicine 2016 Jun;22(6):586-97
Nature medicine 2016 Jun;22(6):586-97
Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
Kowalewska PM, Patrick AL, Fox-Robichaud AE
PloS one 2014;9(9):e104537
PloS one 2014;9(9):e104537
Near-infrared laser adjuvant for influenza vaccine.
Kashiwagi S, Yuan J, Forbes B, Hibert ML, Lee EL, Whicher L, Goudie C, Yang Y, Chen T, Edelblute B, Collette B, Edington L, Trussler J, Nezivar J, Leblanc P, Bronson R, Tsukada K, Suematsu M, Dover J, Brauns T, Gelfand J, Poznansky MC
PloS one 2013;8(12):e82899
PloS one 2013;8(12):e82899
Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.
Scalfone LK, Nel HJ, Gagliardo LF, Cameron JL, Al-Shokri S, Leifer CA, Fallon PG, Appleton JA
Infection and immunity 2013 Apr;81(4):1354-63
Infection and immunity 2013 Apr;81(4):1354-63
Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
Roy RM, Wüthrich M, Klein BS
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2545-52
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2545-52
Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1.
Uchida Y, Takeshita K, Yamamoto K, Kikuchi R, Nakayama T, Nomura M, Cheng XW, Egashira K, Matsushita T, Nakamura H, Murohara T
Diabetes 2012 Jun;61(6):1552-61
Diabetes 2012 Jun;61(6):1552-61
Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization.
Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS
The Journal of biological chemistry 2011 Jun 10;286(23):20991-1001
The Journal of biological chemistry 2011 Jun 10;286(23):20991-1001
MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
Narasaraju T, Ng HH, Phoon MC, Chow VT
American journal of respiratory cell and molecular biology 2010 Jun;42(6):732-43
American journal of respiratory cell and molecular biology 2010 Jun;42(6):732-43
Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice.
Venet F, Huang X, Chung CS, Chen Y, Ayala A
The American journal of pathology 2010 Feb;176(2):764-73
The American journal of pathology 2010 Feb;176(2):764-73
The role for monocyte chemoattractant protein-1 in the generation and function of memory CD8+ T cells.
Wang T, Dai H, Wan N, Moore Y, Dai Z
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2886-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2886-93
Upregulation of VEGF in murine retina via monocyte recruitment after retinal scatter laser photocoagulation.
Itaya M, Sakurai E, Nozaki M, Yamada K, Yamasaki S, Asai K, Ogura Y
Investigative ophthalmology & visual science 2007 Dec;48(12):5677-83
Investigative ophthalmology & visual science 2007 Dec;48(12):5677-83
CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 15;177(4):2651-61
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 15;177(4):2651-61
Role of monocyte chemotactic protein-1/CC chemokine ligand 2 on gamma delta T lymphocyte trafficking during inflammation induced by lipopolysaccharide or Mycobacterium bovis bacille Calmette-Guérin.
Penido C, Vieira-de-Abreu A, Bozza MT, Castro-Faria-Neto HC, Bozza PT
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 15;171(12):6788-94
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 15;171(12):6788-94
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND50 is typically 0.75-3.0 µg/mL.
