Explore
Validate
LearnMAB2791-100
antibody from R&D Systems
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB2791-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL2/JE/MCP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL2/JE/MCP-1 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P13500- Isotype
- IgG
- Antibody clone number
- 23002
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha.
The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
Invasive chronic inflammatory monocyte phenotype in subjects with high HIV-1 viral load.
Rump L, Mattey DL, Kehoe O, Middleton J
Cytokine 2017 Sep;97:133-140
Cytokine 2017 Sep;97:133-140
Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
Bonapace L, Coissieux MM, Wyckoff J, Mertz KD, Varga Z, Junt T, Bentires-Alj M
Nature 2014 Nov 6;515(7525):130-3
Nature 2014 Nov 6;515(7525):130-3
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP 3rd, Zisman DA, Belperio JA
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2008 Jul;8(7):1512-22
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2008 Jul;8(7):1512-22
Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha.
Schell C, Albrecht M, Mayer C, Schwarzer JU, Frungieri MB, Mayerhofer A
Endocrinology 2008 Apr;149(4):1678-86
Endocrinology 2008 Apr;149(4):1678-86
The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G
The American journal of pathology 2007 Dec;171(6):1789-99
The American journal of pathology 2007 Dec;171(6):1789-99
Invasive chronic inflammatory monocyte phenotype in subjects with high HIV-1 viral load.
Pulliam L, Sun B, Rempel H
Journal of neuroimmunology 2004 Dec;157(1-2):93-8
Journal of neuroimmunology 2004 Dec;157(1-2):93-8
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL2/JE/MCP-1 in Human Breast Cancer Tissue. CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB2791) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
