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LearnMAB279-100
antibody from R&D Systems
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB279-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL2/JE/MCP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human (rh) CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL1, 2, 3, 4, 5, 6, 7, 9/10MIP-1 gamma , 11, 12, 17, 19, 20, 22, 24, 25, or rrCCL20.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 24822
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Continuous Delivery of Neutralizing Antibodies Elevate CCL2 Levels in Mice Bearing MCF10CA1d Breast Tumor Xenografts.
Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
Induction of host chemotactic response by Encephalitozoon spp.
Systemic inflammation in nonalcoholic fatty liver disease is characterized by elevated levels of CCL2.
GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
The CC chemokine ligand, CCL2/MCP1, participates in macrophage fusion and foreign body giant cell formation.
Yao M, Smart C, Hu Q, Cheng N
Translational oncology 2017 Oct;10(5):734-743
Translational oncology 2017 Oct;10(5):734-743
Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 11;35(6):2452-64
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 11;35(6):2452-64
Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
Xu W, Joo H, Clayton S, Dullaers M, Herve MC, Blankenship D, De La Morena MT, Balderas R, Picard C, Casanova JL, Pascual V, Oh S, Banchereau J
The Journal of experimental medicine 2012 Sep 24;209(10):1813-23, S1-2
The Journal of experimental medicine 2012 Sep 24;209(10):1813-23, S1-2
Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
International journal of cancer 2010 Mar 1;126(5):1095-108
International journal of cancer 2010 Mar 1;126(5):1095-108
Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Renaud SJ, Sullivan R, Graham CH
Placenta 2009 Apr;30(4):313-9
Placenta 2009 Apr;30(4):313-9
Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
The Journal of pathology 2008 Feb;214(3):328-36
The Journal of pathology 2008 Feb;214(3):328-36
L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
Atherosclerosis 2007 May;192(1):56-66
Atherosclerosis 2007 May;192(1):56-66
Induction of host chemotactic response by Encephalitozoon spp.
Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
Infection and immunity 2007 Apr;75(4):1619-25
Infection and immunity 2007 Apr;75(4):1619-25
Systemic inflammation in nonalcoholic fatty liver disease is characterized by elevated levels of CCL2.
Haukeland JW, Damås JK, Konopski Z, Løberg EM, Haaland T, Goverud I, Torjesen PA, Birkeland K, Bjøro K, Aukrust P
Journal of hepatology 2006 Jun;44(6):1167-74
Journal of hepatology 2006 Jun;44(6):1167-74
GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
Gijsbers K, Gouwy M, Struyf S, Wuyts A, Proost P, Opdenakker G, Penninckx F, Ectors N, Geboes K, Van Damme J
Experimental cell research 2005 Feb 15;303(2):331-42
Experimental cell research 2005 Feb 15;303(2):331-42
The CC chemokine ligand, CCL2/MCP1, participates in macrophage fusion and foreign body giant cell formation.
Kyriakides TR, Foster MJ, Keeney GE, Tsai A, Giachelli CM, Clark-Lewis I, Rollins BJ, Bornstein P
The American journal of pathology 2004 Dec;165(6):2157-66
The American journal of pathology 2004 Dec;165(6):2157-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human CCL2/JE/MCP-1 by Western Blot. Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Human CCL8/ MCP-2 (Catalog # 281-CP), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL13/ MCP-4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/ MCP-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL2/JE/MCP-1 and Neutralization by Human CCL2/JE/MCP-1 Antibody. Recombinant Human CCL2/JE/ MCP-1 (Catalog #279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/ MCP-1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL2/JE/ MCP-1 Monoclonal Antibody (Catalog # MAB279). The ND50 is typically 0.9-4.5 µg/mL.
