F49800

antibody from NSJ Bioreagents

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F49800

Provider

NSJ Bioreagents

Product name

CCL2 Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 62-89 from the human protein was used as the immunogen for this CCL2 antibody.

Description

Purified antibody

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the CCL2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of CCL2 antibody and HeLa lysate.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of CCL2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescence analysis of CCL2 antibody with HeLa cells. Primary Ab was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. Red counterstaining is PI.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human brain tissue with CCL2 antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using CCL2 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.