12-7096-41

antibody from Invitrogen Antibodies

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12-7096-41

Provider

Invitrogen Antibodies

Product name

CCL2 (MCP-1) Monoclonal Antibody (2H5), PE, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Conjugate

Yellow dye

Antibody clone number

2H5

Concentration

5 µL/Test

References

Submitted references

Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis.

Shen C, Lie P, Miao T, Yu M, Lu Q, Feng T, Li J, Zu T, Liu X, Li H
Molecular medicine reports 2015 Jul;12(1):20-30

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Migration of fibroblasts, HUVECs and UC-MSCs in response to UC-CM. (A) A total of 5x10 4 cells were collected and allowed to migrate. Lane 1, UC-CM; lane 2, DMEM; lanes 3-6, in the presence or absence of anti-SDF-1 (20 mu g/ml), anti-MCP-1 (20 mu g/ml) or anti-HGF (20 mu g/ml), respectively. Results are from a representative experiment and are expressed as the mean number of migrated cells in three random fields, scale bar=200 mu m. Cells that crossed the matrigel membrane were stained with crystal violet (magnification, x40). (B) Graphical presentation of the quantified data, presented as the number of migrated cells and expressed as the mean +- standard error of the mean. HUVECs, human umbilical vein endothelial cells; UC-MSCs, umbilical cord mesenchymal stem cells; UC-CM, UC-MSCs conditioned medium; DMEM, Dulbecco's modified Eagle's medium; SDF-1, stromal cell-derived factor 1; MCP-1, monocyte chemotactic protein 1; HGF, hepatocyte growth factor.

Conjugate

Yellow dye