17-7099-81
antibody from Invitrogen Antibodies
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 17-7099-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.
- Antibody clone number
- 5D3-F7
- Concentration
- 0.2 mg/mL
Submitted references Respiratory syncytial virus G protein CX3C motif impairs human airway epithelial and immune cell responses.
The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic sclerosis.
Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation.
Chirkova T, Boyoglu-Barnum S, Gaston KA, Malik FM, Trau SP, Oomens AG, Anderson LJ
Journal of virology 2013 Dec;87(24):13466-79
Journal of virology 2013 Dec;87(24):13466-79
The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic sclerosis.
Karrer S, Bosserhoff AK, Weiderer P, Distler O, Landthaler M, Szeimies RM, Müller-Ladner U, Schölmerich J, Hellerbrand C
The Journal of investigative dermatology 2005 Jan;124(1):92-8
The Journal of investigative dermatology 2005 Jan;124(1):92-8
Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation.
Piemonti L, Leone BE, Nano R, Saccani A, Monti P, Maffi P, Bianchi G, Sica A, Peri G, Melzi R, Aldrighetti L, Secchi A, Di Carlo V, Allavena P, Bertuzzi F
Diabetes 2002 Jan;51(1):55-65
Diabetes 2002 Jan;51(1):55-65
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stimulated with LPS for 5 hours in the presence of Monensin Solution (Product # 00-4505-51). The cells were intracellularly stained with Anti-Human CD11c PerCP-eFluor® 710 (Product # 46-0116-42) and 0.03 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or 0.03 µg of Anti-Human CCL2 (MCP-1) APC (right) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the monocyte gate were used for analysis.